Transcriptomic profile of tobacco in response to Alternaria longipes and Alternaria alternata infections
Transcriptomic profile of tobacco in response to Alternaria longipes and Alternaria alternata infections
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DOI：
10.1038/srep25635
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发表时间：
2016-05-09
影响因子：
4.6
通讯作者：
Dong, Yang
中科院分区：
文献类型：
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作者：
Duan, Shengchang;Ma, Xiao;Dong, Yang
烟草赤星病是由烟草赤星病菌引起的危害最大的病害之一，给烟草生产造成重大损失。然而，人们对烟草对这种真菌感染的系统反应知之甚少。为了填补这一知识空白，在V2和NC89品种中，在3个不同的时间点接种长链格孢菌(AL)或交链格孢菌(AA)后，获得了烟草叶片转录的从头组装。我们研究了每个品种-病原菌组合的基因表达谱，并鉴定了8个在所有组合之间共享的差异表达基因。对差异表达基因的基因本体丰富分析揭示了真菌感染过程中的关键成分，包括基因表达调控(GO：0010468)、核糖核酸代谢过程调控(GO：0051252)、四吡咯结合(GO：0046906)和外包被结构(GO：0030312)。对持续上调/下调的基因和抗性基因的进一步分析表明，真菌感染时的基因表达谱取决于特定的品种和病原菌。综上所述，本研究为植物-病原菌相互作用的研究奠定了坚实的基础，对病害防治和分子育种具有重要意义。
Tobacco brown spot caused by Alternaria fungal species is one of the most damaging diseases, and results in significant yield losses. However, little is known about the systematic response of tobacco to this fungal infection. To fill this knowledge gap, de novo assemblies of tobacco leaf transcriptomes were obtained in cultivars V2 and NC89 after the inoculation of either Alternaria longipes (AL) or Alternaria alternata (AA) at three different time points. We studied the gene expression profile of each cultivar-pathogen combination, and identified eight differentially expressed genes shared among all combinations. Gene ontology enrichment analysis of the differentially expressed genes revealed key components during the fungal infection, which included regulation of gene expression (GO: 0010468), regulation of RNA metabolic process (GO: 0051252), tetrapyrrole binding (GO: 0046906), and external encapsulating structure (GO: 0030312). Further analyses of the continuously upregulated/downregulated genes and the resistance genes demonstrated that the gene expression profile upon fungal infection was contingent on the specific cultivar and pathogen. In conclusion, this study provides a solid foundation for the investigation of plant-pathogen interaction, and is of great importance for disease prevention and molecular breeding.