Caenorhabditis elegans as a model in developmental toxicology.
Caenorhabditis elegans as a model in developmental toxicology.
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DOI：
10.1007/978-1-61779-867-2_3
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Freedman, Jonathan H
中科院分区：
文献类型：
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作者：
Boyd, Windy A;Smith, Marjolein V;Freedman, Jonathan H
许多实际优势使线虫秀丽隐杆线虫成为遗传和发育生物学研究的有用模型。这些相同的优点，加上疾病和应激反应途径的保护、突变体和转基因菌株的可用性以及丰富的生物信息，导致线虫在毒理学研究中的使用增加。尽管研究线虫发育毒理学机制的潜力是有希望的，但用于鉴定影响线虫的化合物的胚胎和幼虫生长测试仍然是线虫在发育毒理学中的主要用途。在这里，我们描述了使用 COPAS Biosort 流式细胞仪进行中和高通量筛选的线虫幼虫生长和发育测定，并提供了数据和后续分析的描述。
A number of practical advantages have made the nematode Caenorhabditis elegans a useful model for genetic and developmental biological research. These same advantages, along with conservation of disease and stress response pathways, availability of mutant and transgenic strains, and wealth of biological information, have led to the increased use of C. elegans in toxicological studies. Although the potential to study the mechanisms of developmental toxicology in C. elegans is promising, embryonic and larval growth tests to identify compounds that affect the nematode have remained the primary use of C. elegans in developmental toxicology. Here, we describe a C. elegans larval growth and development assay for medium and high throughput screening using the COPAS Biosort flow cytometer and provide descriptions of the data and subsequent analysis.