Translational halt during elongation caused by G-quadruplex formed by mRNA
Translational halt during elongation caused by G-quadruplex formed by mRNA
复制标题
DOI：
10.1016/j.ymeth.2013.05.026
复制
发表时间：
2013-11-01
期刊：
影响因子：
4.8
通讯作者：
Sugimoto, Naoki
中科院分区：
文献类型：
--
作者：
Endoh, Tamaki;Kawasaki, Yu;Sugimoto, Naoki
mRNA形成影响基因表达的各种二级和三级结构。虽然在mRNA的非翻译区（UTR）中形成的抑制翻译的结构已被表征，但开放阅读框（ORF）中的稳定mRNA结构也可能导致翻译停止或缓慢的翻译延伸。我们以前建立了一种方法，称为同步翻译测定，使单周转翻译延长的时间过程分析。在该方法中，可以忽略翻译起始，这是翻译过程的速率决定步骤，因为在翻译延伸从mRNA的特定位置重新开始之前，所有核糖体在该位置上同步。在本文中，我们使用的同步翻译试验，以评估的G-四链体结构位于mRNA的ORF内的翻译停止的不同位置的影响。(C)2013 Elsevier Inc. All rights reserved.
mRNA forms various secondary and tertiary structures that affect gene expression. Although structures formed in the untranslated regions (UTRs) of mRNAs that inhibit translation have been characterized, stable mRNA structures in open reading frames (ORFs) may also cause translational halt or slow translation elongation. We previously established a method, termed a synchronized translation assay, that enables time course analysis of single turnover translation elongation. In this method, translation initiation, which is a rate determining step of the translation procedure, can be ignored because all ribosomes are synchronized on a specific position of mRNA before translation elongation is restarted from this position. In this paper, we used the synchronized translation assay to evaluate the effects of a G-quadruplex structure located at various positions within the mRNA ORF on translational halt. (C) 2013 Elsevier Inc. All rights reserved.