Increase in dicentric chromosome formation after a single CT scan in adults.
Increase in dicentric chromosome formation after a single CT scan in adults.
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DOI：
10.1038/srep13882
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发表时间：
2015-09-09
影响因子：
4.6
通讯作者：
Sakai A
中科院分区：
文献类型：
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作者：
Abe Y;Miura T;Yoshida MA;Ujiie R;Kurosu Y;Kato N;Katafuchi A;Tsuyama N;Ohba T;Inamasu T;Shishido F;Noji H;Ogawa K;Yokouchi H;Kanazawa K;Ishida T;Muto S;Ohsugi J;Suzuki H;Ishikawa T;Kamiya K;Sakai A
有报道称，儿童CT扫描后患白血病和脑瘤的风险过高。我们在CT扫描前后进行了双着丝粒染色体分析(DCAs)，以评估低剂量电离辐射对染色体的影响。10例患者在CT扫描前后采集外周血(PB)淋巴细胞。通过Giemsa染色和着丝粒荧光原位杂交(着丝粒FISH)分析1,000或2,000个中期染色体，进行DCA。将DIC形成的增量与使用计算剂量学系统、WAZA-ARI和CT扫描的剂量长度乘积(DLP)计算的有效辐射剂量进行比较。单次CT扫描后双着丝粒染色体(DIC)形成显著增加，所有患者均发现DIC形成增加。用Giemsa染色分析2,000个中期的DIC形成增量与用着丝粒FISH分析的2,000个中期的增量之间有很好的相关性。然而，DIC形成的增加与有效辐射剂量之间没有相关性。因此，这些结果表明，一次CT扫描可能会导致染色体分裂，我们建议在低剂量辐射暴露的情况下，对DCA进行Giemsa染色或着丝粒FISH分析2,000个或更多的中期染色体。
Excess risk of leukemia and brain tumors after CT scans in children has been reported. We performed dicentric chromosome assay (DCAs) before and after CT scan to assess effects of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) lymphocytes were collected from 10 patients before and after a CT scan. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). The increment of DIC formation was compared with effective radiation dose calculated using the computational dosimetry system, WAZA-ARI and dose length product (DLP) in a CT scan. Dicentric chromosome (DIC) formation increased significantly after a single CT scan, and increased DIC formation was found in all patients. A good correlation between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH was observed. However, no correlation was observed between the increment of DIC formation and the effective radiation dose. Therefore, these results suggest that chromosome cleavage may be induced by one CT scan, and we recommend 2,000 or more metaphases be analyzed in Giemsa staining or Centromere-FISH for DCAs in cases of low-dose radiation exposure.