Protective effects of trapidil in ischemia-reperfusion injury due to testicular torsion and detorsion: An experimental study
Protective effects of trapidil in ischemia-reperfusion injury due to testicular torsion and detorsion: An experimental study
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DOI：
10.1111/j.1442-2042.2006.01355.x
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发表时间：
2006-05-01
影响因子：
2.6
通讯作者：
Kaya, M
中科院分区：
文献类型：
--
作者：
Somuncu, S;Cakmak, M;Kaya, M
目的：方法：采用青春期前白化病大鼠40只，观察曲匹地尔对睾丸扭转和复位所致缺血再灌注损伤的预防作用。IR组通过旋转左侧睾丸2 h造成扭转，然后通过解扭睾丸进行扭转复位。4 h后行双侧睾丸切除术。研究组在扭转2 h后，于扭转前1h单次给予曲匹地尔。4 h后行双侧睾丸切除术。假手术组行假手术。在假手术加曲匹地尔组中，进行假手术并单次给予曲匹地尔。检测睾丸组织丙二醛（MDA）、一氧化氮（NO）和总巯基（T-SH）含量。结果：IR组左侧睾丸组织中NO、MDA含量显著高于对照组、假手术组和假手术+曲匹地尔组（P < 0.05、P < 0.001和P < 0.001）。左侧睾丸NO和MDA含量在研究组、假手术组和假手术+曲匹地尔组之间差异无统计学意义（P > 0.05）。实验组左侧睾丸T-SH水平显著高于IR组、假手术组和假手术+曲匹地尔组（P < 0.05）。IR组（左侧睾丸）I级间质损伤为30%（3/10），2级损伤为60%（6/10），3级损伤为10%（1/10）。实验组（左侧睾丸）I级间质损伤30%（3/10），无损伤70%（7/10）。结论：曲匹地尔可减少扭转和复位过程中氧自由基的生成，减轻扭转侧睾丸组织病理学损伤。
Objective: We aimed to detect the preventive effects of trapidil in ischemia reperfusion (IR) injury due to testicular torsion and detorsion.Methods: Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose I h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination.Results: The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P < 0.05 P < 0.001 and P < 0.001, respectively). A statistical difference was not found among Study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P > 0.05). The T-SH level in the study group was significantly higher than in the IR. sham and sham plus trapidil groups in left testis P < 0.05). In the IR group (left testis), grade I interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade I interstitial injury was 30% (3/10) and there was no injury in 70% (7/10).Conclusion: Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.