Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA
Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA
复制标题
miR-318 microRNA 对果蝇卵子发生过程中模式形成和基因扩增的调节。
DOI：
10.1534/genetics.115.174748
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发表时间：
2015-05-01
期刊：
影响因子：
3.3
通讯作者：
Cohen, Stephen M.
中科院分区：
文献类型：
--
作者：
Ge, Wanzhong;Deng, Qiannan;Cohen, Stephen M.
上皮发育过程中的模式形成需要多种信号通路的协调。在这里，我们研究卵巢富集的miRNA，miR-318，在果蝇卵子发生过程中的上皮发育的功能。mir-318母体功能丧失突变体是雌性不育的，并且产下具有异常形态的卵。去除mir-318破坏了背前卵泡细胞的模式，导致异常的背侧附属物。mir-318突变雌性也由于受损的绒毛膜基因扩增而产生薄而易碎的蛋壳。我们提供的证据表明，蜕皮激素信号通路激活miR-318的表达，miR-318与Tramtrack 69合作，以控制从内循环到绒毛膜基因扩增的转换在滤泡上皮分化。miR-318在卵子发生中的多种功能说明了miRNA在维持细胞命运和促进雌性滤泡上皮发育转变中的重要性。
Pattern formation during epithelial development requires the coordination of multiple signaling pathways. Here, we investigate the functions of an ovary-enriched miRNA, miR-318, in epithelial development during Drosophila oogenesis. mir-318 maternal loss-of-function mutants were female-sterile and laid eggs with abnormal morphology. Removal of mir-318 disrupted the dorsal-anterior follicle cell patterning, resulting in abnormal dorsal appendages. mir-318 mutant females also produced thin and fragile eggshells due to impaired chorion gene amplification. We provide evidence that the ecdysone signaling pathway activates expression of miR-318 and that miR-318 cooperates with Tramtrack69 to control the switch from endocycling to chorion gene amplification during differentiation of the follicular epithelium. The multiple functions of miR-318 in oogenesis illustrate the importance of miRNAs in maintaining cell fate and in promoting the developmental transition in the female follicular epithelium.