A Localized Surface Plasmon Resonance Biosensor Based on Integrated Controllable Au2S/AuAgS-Coated Gold Nanorods Composite
A Localized Surface Plasmon Resonance Biosensor Based on Integrated Controllable Au2S/AuAgS-Coated Gold Nanorods Composite
复制标题
DOI：
10.1007/s11468-010-9162-7
复制
发表时间：
2011-03
期刊：
影响因子：
3
通讯作者：
Haowen Huang;Xuanyong Liu;Bo Liao;P. Chu
中科院分区：
文献类型：
--
作者：
Haowen Huang;Xuanyong Liu;Bo Liao;P. Chu
在这项工作中，我们证明了基于Au2S/AuAgS包覆金纳米棒(Au2S/AuAgS包覆纳米棒)的局域表面等离子体共振(LSPR)的精细光学性质可以开发出一种简单而灵敏的生物传感器，并且羊抗人IgG探针可以检测到低至0.2 nm的人免疫球蛋白。此外，我们还介绍了将固定在玻片上的Au2S/AuAgS包覆的GNR与孤立的Au2S/AuAgS包覆的GNR以液体的形式结合在一起的集成式LSPR生物传感器。通过单个颗粒附近折射率的变化引起的直接光谱变化来检测靶结合。集成的LSPR光学生物传感器无需标签，成本效益高，易于制造，只需一台可见光/近红外光谱仪即可进行检测。此外，还研究了两种纳米棒在集成LSPR生物传感器中的相互影响。单独实验的结果表明，孤立或集成形式的纳米棒表现出相似的行为。
In this work, we have demonstrated that the exquisite optical properties based on localized surface plasmon resonance (LSPR) of Au2S/AuAgS-coated gold nanorods (Au2S/AuAgS-coated GNRs) can be utilized to develop a simple and sensitive biosensor, and goat anti-human IgG can be detected by the human IgG probe as low as 0.2 nM. Moreover, we introduce an integrated LSPR biosensor constructed by integrating Au2S/AuAgS-coated GNRs immobilized on glass slide and isolated Au2S/AuAgS-coated GNRs in the form of liquid. The detection of target binding was performed via direct spectral changes induced by changes of refractive index in the vicinity of individual particles. The integrated LSPR optical biosensor is label-free, cost-effective, and easy to fabricate and requires only a visible/near-infrared spectrometer for detection purposes. Additionally, the investigation on the mutual influence of the two types of nanorods in the integrated LSPR biosensor was performed. The results of separate experiments indicate that the nanorods in the form of isolate or in integrated exhibit a similar behavior.