Secretory component production by polarized epithelial cells from the human female reproductive tract
Secretory component production by polarized epithelial cells from the human female reproductive tract
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DOI：
10.3109/08820139809089454
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发表时间：
1998-01-01
影响因子：
2.8
通讯作者：
Wira, CR
中科院分区：
文献类型：
--
作者：
Fahey, JV;Humphrey, SL;Wira, CR
在粘膜表面，多聚IG受体（pIgR）负责跨上皮细胞转运多聚伊加。本研究的目的是确定女性生殖道的正常上皮细胞是否形成紧密连接并产生分泌成分，即pIgR的外部结构域。来自月经周期不同阶段和绝经后妇女的子宫、宫颈和阴道组织用于制备纯化的上皮细胞片，将其在细胞室中培养。测量跨上皮阻力，并测定顶侧和基底侧隔室的培养基中的分泌成分。子宫上皮细胞产生的分泌成分优先积聚在顶端室，并与跨上皮阻力增加相关。生长需要以1x 10（6）个细胞/cm（2）基质包被细胞室的上皮片接种。子宫颈内膜和外宫颈上皮细胞，但不是阴道，也表现出优先生产和释放的分泌成分到顶室。总之，正常女性生殖道上皮细胞生长汇合，极化并产生分泌成分。我们的研究结果表明，子宫和宫颈上皮细胞在控制伊加从组织转胞吞到分泌物中起着关键的调节作用。
At mucosal surfaces, the polymeric Ig receptor (pIgR) is responsible for transporting polymeric IgA across epithelial cells. The purpose of this study was to determine whether normal epithelial cells from the female reproductive tract form tight junctions and produce secretory component, the external domain of the pIgR. Uterine, cervical and vaginal tissues from women at different stages of the menstrual cycle and following menopause were used to prepare purified epithelial cell sheets, which were cultured in cell chambers. Transepithelial resistance was measured and the media from apical and basolateral compartments assayed for secretory component. Secretory component produced by uterine epithelial cells accumulated preferentially in apical compartment and correlated with increased transepithelial resistance. Seeding as epithelial sheets at 1x10(6)cells/cm(2) of matrix coated cell chambers was required for growth. Epithelial cells from endo-cervix and ecto-cervix, but not the vagina, also showed preferential production and release of secretory component into the apical chamber. In conclusion, normal epithelial cells from the human female reproductive tract grow to confluence, become polarized and produce secretory component. Our results suggest that uterine and cervical epithelial cells play a key regulatory role in the control of IgA transcytosis from tissue into secretions.