Multimode nonlinear optical imaging of the dermis in ex vivo human skin based on the combination of multichannel mode and Lambda mode
Multimode nonlinear optical imaging of the dermis in ex vivo human skin based on the combination of multichannel mode and Lambda mode
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基于多通道模式与Lambda模式相结合的离体人体皮肤真皮多模非线性光学成像
DOI：
10.1364/oe.14.007810
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发表时间：
2006-08-21
期刊：
影响因子：
3.8
通讯作者：
Zhao, Jingjun
中科院分区：
文献类型：
--
作者：
Zhuo, Shuangmu;Chen, Jianxin;Zhao, Jingjun
提出了一种基于多通道模式和Lambda模式相结合的多模非线性光学成像技术，用于人体真皮的研究。我们的研究结果表明，该技术不仅提高了细胞外基质（ECM）的结构蛋白的图像对比度，而且还提供了一种图像引导的光谱分析方法，以识别细胞和ECM的内在成分，包括胶原蛋白，弹性蛋白，NAD（P）H和黄素。通过多通道模式和Lambda模式的串联组合使用，获得了深度双光子激发荧光（TPEF）和二次谐波产生（SHG）成像以及TPEF/SHG信号深度依赖性衰减，可以为获得定量组织结构和生化信息提供灵敏的工具。这些结果表明，该技术有可能提供更准确的信息，确定组织的生理和病理状态。(c)2006年美国光学学会。
A Multimode nonlinear optical imaging technique based on the combination of multichannel mode and Lambda mode is developed to investigate human dermis. Our findings show that this technique not only improves the image contrast of the structural proteins of extracellular matrix (ECM) but also provides an image-guided spectral analysis method to identify both cellular and ECM intrinsic components including collagen, elastin, NAD(P) H and flavin. By the combined use of multichannel mode and Lambda mode in tandem, the obtained in-depth two photon-excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging and TPEF/SHG signals depth-dependence decay can offer a sensitive tool for obtaining quantitative tissue structural and biochemical information. These results suggest that the technique has the potential to provide more accurate information for determining tissue physiological and pathological states. (c) 2006 Optical Society of America.