GROWTH AND NUCLEIC ACID SYNTHESIS IN SYNCHRONOUSLY DIVIDING POPULATIONS OF HELA CELLS
GROWTH AND NUCLEIC ACID SYNTHESIS IN SYNCHRONOUSLY DIVIDING POPULATIONS OF HELA CELLS
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DOI：
10.1016/0014-4827(63)90306-9
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发表时间：
1963-01-01
影响因子：
3.7
通讯作者：
TOLMACH, LJ
中科院分区：
文献类型：
--
作者：
TERASIMA, T;TOLMACH, LJ
人HeLa S3细胞生长的同步化已经通过纯选择性方法实现，该方法利用了有丝分裂期间细胞与生长表面的微弱结合。收获的群体是85-90%有丝分裂的，并且具有不能与随机群体中的相应参数区分的生长参数。使用放射自显影方法，发现DNA合成周期各阶段的平均持续时间如下：有丝分裂后（G1），41%的中位无丝分裂时间; DNA合成（S），46%;有丝分裂前（G2），11%;有丝分裂，3%。在S期DNA合成的平均速率被发现通过一个最大值大约中途通过该阶段，并有迹象表明，在单个细胞的合成速率的行为类似。RNA合成的平均速率在间期至少增加了三倍。在有丝分裂细胞中没有检测到RNA合成。发现平均细胞体积以非线性方式增加两倍。种群的去荒漠化似乎主要发生在G1期。
Synchronization of growth of human HeLa S3 cells has been effected by a purely selective method which takes advantage of the tenuous binding of cells to the growth surface during mitosis. The harvested populations are 85-90% mitotic, and have growth parameters that can not be distinguished from the corresponding parameters in random populations. The following average durations were found for the phases of the DNA synthetic cycle, using autoradiographic methods: post-mitotic (G1), 41% of the median intermitotic time; DNA synthetic (S), 46%; pre-mitotic (G2), 11%; and mitotic, 3%. The average rate of DNA synthesis during the S phase was found to pass through a maximum approximately midway through that phase, and there are indications that the rate of synthesis in individual cells behaves similarly. The average rate of RNA synthesis showed at least a threefold increase during interphase. No RNA synthesis was detected in mitotic cells. The average cell volume was found to increase two-fold in a non-linear fashion. Desynchronization of the population appears to arise largely during the G1 phase.