Identification of Potential Candidate Genes of Oral Cancer in Response to Chronic Infection With Porphyromonas gingivalis Using Bioinformatical Analyses
Identification of Potential Candidate Genes of Oral Cancer in Response to Chronic Infection With Porphyromonas gingivalis Using Bioinformatical Analyses
复制标题
利用生物信息分析鉴定口腔癌对牙龈卟啉单胞菌慢性感染的潜在候选基因
DOI：
10.3389/fonc.2019.00091
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发表时间：
2019-02-21
影响因子：
4.7
通讯作者：
Pan, Yaping
中科院分区：
文献类型：
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作者：
Geng, Fengxue;Wang, Qingxuan;Pan, Yaping
近年来的研究表明，慢性牙周炎、牙龈卟啉单胞菌与癌症之间存在着密切的关系。然而，对牙龈卟啉单胞菌慢性感染作出反应而发生变化并可能导致口腔癌的宿主基因在很大程度上仍然未知。在本研究中，我们的目的是全面分析从慢性感染模型的永生化口腔上皮细胞，持续暴露于牙龈卟啉单胞菌15周获得的微阵列数据。使用蛋白质-蛋白质相互作用（PPI）网络和不相容性通路分析（IPA），我们鉴定了枢纽基因、主要生物学过程、上游调节因子和可能参与肿瘤起始和进展的基因。我们还验证了基因表达，并证明了来自头颈癌临床样本的枢纽基因的遗传改变。总之，我们利用生物信息学方法鉴定IL 6、STAT 1、林恩、BDNF、C3、CD 274、PDCD 1 LG 2和CXCL 10作为可能促进口腔鳞状细胞癌（OSCC）（最常见的头颈部鳞状细胞癌（HNSCC）类型）预防和治疗的潜在候选基因。
Recent investigations revealed the relationship between chronic periodontitis, Porphyromonas gingivalis and cancer. However, host genes that change in response to chronic infection with P. gingivalis and may contribute to oral cancer have remained largely unknown. In the present study, we aimed to comprehensively analyze microarray data obtained from the chronic infection model of immortalized oral epithelial cells that were persistently exposed to P. gingivalis for 15 weeks. Using protein-protein interaction (PPI) networks and Ingenuity Pathway Analysis (IPA), we identified hub genes, major biological processes, upstream regulators and genes potentially involved in tumor initiation and progression. We also validated gene expression and demonstrated genetic alteration of hub genes from clinical samples of head and neck cancer. Overall, we utilized bioinformatical methods to identify IL6, STAT1, LYN, BDNF, C3, CD274, PDCD1LG2, and CXCL10 as potential candidate genes that might facilitate the prevention and treatment of oral squamous cell carcinoma (OSCC), the most common type of head and neck squamous cell carcinoma (HNSCC).