High conservation of transcription factor binding and evidence for combinatorial regulation across six Drosophila species
High conservation of transcription factor binding and evidence for combinatorial regulation across six Drosophila species
复制标题
DOI：
10.1038/ng.808
复制
发表时间：
2011-05-01
期刊：
影响因子：
30.8
通讯作者：
Zeitlinger, Julia
中科院分区：
文献类型：
--
作者：
He, Qiye;Bardet, Anais F.;Zeitlinger, Julia
某些转录因子的结合已显示出在密切相关的物种之间显着差异。在这里，我们表明，在六种果蝇物种中，发育转录因子扭曲的结合高度保守，揭示了其增强子的强大功能约束。保守的结合与扭曲及其伴侣的序列基序相关，允许从头发现其组合结合。它还包括在检测极限附近的10,000多个低占用位点，这往往会标记后来发育阶段的增强子。这些结果表明，发展增强剂可以在进化上受到高度限制，这可能是由于其复杂的组合性质。
The binding of some transcription factors has been shown to diverge substantially between closely related species. Here we show that the binding of the developmental transcription factor Twist is highly conserved across six Drosophila species, revealing strong functional constraints at its enhancers. Conserved binding correlates with sequence motifs for Twist and its partners, permitting the de novo discovery of their combinatorial binding. It also includes over 10,000 low-occupancy sites near the detection limit, which tend to mark enhancers of later developmental stages. These results suggest that developmental enhancers can be highly evolutionarily constrained, presumably because of their complex combinatorial nature.