Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding
Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding
复制标题
DOI：
10.1016/s0092-8674(03)00643-3
复制
发表时间：
2003-08-22
期刊：
影响因子：
64.5
通讯作者：
Shur, BD
中科院分区：
文献类型：
--
作者：
Ensslin, MA;Shur, BD
我们报告的识别SED 1，所需的蛋白质小鼠精子结合到卵透明质酸。SED 1与一小组分泌的细胞基质粘附蛋白同源，这些蛋白含有Notch样EGF重复序列和盘状蛋白/F5/8 C型结构域。SED 1在生精细胞中表达，并由附睾头的起始段分泌，导致SED 1定位于覆盖顶体的精子质膜上。SED 1特异性结合未受精卵的透明质酸，但不结合受精卵的透明质酸。重组SED 1和抗SED 1抗体竞争性抑制精卵结合，含有盘状蛋白/C结构域的截短SED 1蛋白也是如此。SED 1基因缺失的雄性不育，其精子在体外不能与卵细胞结合。这些研究表明，Notch样EGF和discoidin/C结构域，促进各种细胞相互作用的蛋白质基序，也参与配子识别。
We report the identification of SED1, a protein required for mouse sperm binding to the egg zona pellucida. SED1 is homologous to a small group of secreted cell-matrix adhesive proteins that contain Notch-like EGF repeats and discoidin/F5/8 type C domains. SED1 is expressed in spermatogenic cells and is secreted by the initial segment of the caput epididymis, resulting in SED1 localization on the sperm plasma membrane overlying the acrosome. SED1 binds specifically to the zona pellucida of unfertilized oocytes, but not to the zona of fertilized eggs. Recombinant SED1 and anti-SED1 antibodies competitively inhibit sperm-egg binding, as do truncated SED1 proteins containing a discoidin/C domain. SED1 null males are subfertile and their sperm are unable to bind to the egg coat in vitro. These studies illustrate that Notch-like EGF and discoidin/C domains, protein motifs that facilitate a variety of cellular interactions, participate in gamete recognition as well.