Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes
Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes
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DOI：
10.1099/ijs.0.03048-0
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Martínez-Murcia, AJ
中科院分区：
文献类型：
--
作者：
Soler, L;Yáñez, MA;Martínez-Murcia, AJ
使用编码sigma（70）因子的rpoD序列，对气单胞菌属的所有已知种，特别是牛气单胞菌和杀鲑气单胞菌的70个菌株进行了系统发育关系研究。该分析与gyrB序列互补，该序列已被证明可用于确定属中的系统发育关系。rpoD和gyrB的核苷酸序列显示，这两个基因具有相似的取代率（< 2%）和相似的可变位置数（rpoD为34%，gyrB为32%）。通过分析rpoD，gyrB和两种基因的组合的菌株分组与迄今为止描述的所有气单胞菌属物种的分类组织一致。然而，同时分析两个时钟提高了可靠性和权力，以区分，特别是密切相关的类群。在种间水平上，gyrB对气单胞菌HG 11/气单胞菌encheleia和气单胞菌veronii/嗜酸气单胞菌AeromonasculicolalAeromonasallosaccharophila有较好的区分能力，而rpoD对A. salmonicida从A.野兽rpoD的分析提供了初步证据，明确的系统发育分化后两个物种。
The phylogenetic relationships of all known species of the genus Aeromonas, and especially Aeromonas bestiarum and Aeromonas salmonicida, were investigated on 70 strains using the rpoD sequence, which encodes the sigma(70) factor. This analysis was complemented with the sequence of gyrB, which has already proven useful for determining the phylogenetic relationships in the genus. Nucleotide sequences of rpoD and gyrB showed that both genes had similar substitution rates ( < 2%) and a similar number of variable positions (34% for rpoD versus 32% for gyrB). Strain groupings by analysis of rpoD, gyrB and a combination of both genes were consistent with the taxonomic organization of all Aeromonas species described to date. However, the simultaneous analysis of both clocks improved the reliability and the power to differentiate, in particular, closely related taxa. At the inter-species level, gyrB showed a better resolution for differentiating Aeromonas sp. HG11/Aeromonas encheleia and Aeromonas veronii/Aeromonas culicicolal Aeromonas allosaccharophila, while rpoD more clearly differentiated A. salmonicida from A. bestiarum. The analysis of rpoD provided initial evidence for clear phylogenetic divergence between the latter two species.