Characterization of cDNAs of the human pregnancy-specific beta 1-glycoprotein family, a new subfamily of the immunoglobulin gene superfamily.
Characterization of cDNAs of the human pregnancy-specific beta 1-glycoprotein family, a new subfamily of the immunoglobulin gene superfamily.
复制标题
人类妊娠特异性 β1-糖蛋白家族（免疫球蛋白基因超家族的一个新亚家族）的 cDNA 的表征。
DOI：
10.1021/bi00463a030
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Chan,WY
中科院分区：
文献类型：
--
作者：
Zheng,QX;Tease,LA;Shupert,WL;Chan,WY
摘要：从人胎盘cDNA文库中分离到3个高度同源的人妊娠特异性01-糖蛋白（SP1）。这些cdna在其编码序列中具有> - 90%的核苷酸同源性，> - 79%的编码氨基酸同源性。由这些cdna编码的蛋白质与癌胚抗原家族成员非常相似，含有重复结构域、保守的二硫桥和免疫球蛋白基因超家族典型的0层结构。然而，SP1蛋白之间高度的序列同源性和相对较小的糖基化程度表明它们作为一个独特的家族而不是CEA家族的成员存在。在胎盘中存在可溶性和潜在的膜结合形式的SP1蛋白。特异探针Northern blot分析证实，人足月胎盘中存在多种mRNA的表达。
Revised Manuscript Received November 2, 1989 abstract: Threehighly homologous cDNAs encoding human pregnancy-specific 01-glycoprotein (SP1) were isolated from a human placental cDNA library. These cDNAs share> 90% nucleotide homology in their coding sequences, and> 79% of the encoded amino acids are homologous. Proteins encoded by these cDNAs are very similar to members of the carcinoembryonic antigen family and contain repeating domains, conserved disulfide bridges, and 0-sheet structure typical of the immunoglobulin gene superfamily. However, the high degree of sequence homology and relatively lesser degree of glycosylation among the SP1 proteins suggest that theyexist as a unique family instead of being members of the CEA family. Both soluble and potentially membrane-bound forms of SP1 proteins were present in the placenta. Northern blot analysis using specific probes confirmed the expression of multiple mRNA species in human term placenta.