Bacteriophage Administration Reduces the Concentration of Listeria monocytogenes in the Gastrointestinal Tract and Its Translocation to Spleen and Liver in Experimentally Infected Mice.
Bacteriophage Administration Reduces the Concentration of Listeria monocytogenes in the Gastrointestinal Tract and Its Translocation to Spleen and Liver in Experimentally Infected Mice.
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DOI：
10.1155/2010/624234
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发表时间：
2010
影响因子：
3.4
通讯作者：
Sulakvelidze A
中科院分区：
文献类型：
--
作者：
Mai V;Ukhanova M;Visone L;Abuladze T;Sulakvelidze A
为了研究噬菌体补充在减少病原体数量中的功效，在口服感染李斯特菌之前，通过口服管饲法用单核细胞增生李斯特菌噬菌体制剂（命名为ListShield）处理小鼠。单核细胞增多症L.在经噬菌体处理的小鼠中，肝、脾和肠中的单核细胞增多症显著低于对照小鼠（P <0.05）。噬菌体和抗生素治疗在降低L.单核细胞增多症在受感染的小鼠的内部器官。然而，在对照组和驱虫剂治疗组中检测到的显著体重减轻在感染的ListShield治疗的小鼠中未观察到。长期（90天），每两周一次用ListShield治疗未感染的小鼠，没有引起大肠微生物群的可检测变化或健康状况的有害变化。我们的数据支持使用噬菌体控制L.小鼠单核细胞增多症，而不影响肠道微生物群组成。
To investigate the efficacy of phage supplementation in reducing pathogen numbers, mice were treated via oral gavage with a Listeria monocytogenes phage preparation (designated ListShield) before being orally infected with L. monocytogenes. The concentrations of L. monocytogenes in the liver, spleen, and intestines were significantly lower (P < .05) in the phage-treated than in the control mice. Phage and antibiotic treatments were similarly effective in reducing the levels of L. monocytogenes in the internal organs of the infected mice. However, the significant weight loss detected in the control and antibiotic-treated groups was not observed in the infected, ListShield-treated mice. Long-term (90 days), biweekly treatment of uninfected mice with ListShield did not elicit detectable changes in the microbiota of their large intestines or deleterious changes in their health. Our data support the potential feasibility of using bacteriophages to control proliferation of L. monocytogenes in mice without affecting commensal microbiota composition.