Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
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依达拉奉可预防链脲佐菌素诱导的糖尿病小鼠的视网膜损伤
DOI：
10.1371/journal.pone.0099219
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Yuan D;Xu Y;Hang H;Liu X;Chen X;Xie P;Yuan S;Zhang W;Lin X;Liu Q
Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one), a free radical scavenger, is used for the clinical treatment of retinal injury. In this study, we investigated the protective effects of edaravone against diabetic retinal damage in the mouse. Diabetic retinopathy in the mouse was induced by injection of streptozotocin. Edaravone was given once-daily and was intraperitoneally (i.p.) treated at a dose of 3 mg/kg from streptozotocin injection to 4 weeks after onset of diabetes. Retinal ganglion cells (RGCs) damage was evaluated by recording the pattern electroretinogram (ERG). RGCs damage was also detected by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and the levels of reactive oxygen species (ROS) were determined fluorometrically. The expressions of phosporylated-ERK1/2, BDNF, and caspase-3 were determined by Western blot analysis. Retinal levels of ROS, phosphorylated ERK1/2, and cleaved caspase-3 were significantly increased, whereas the expression of BDNF was significantly decreased in the retinas of diabetic mice, compared to nondiabetic mice. Administration of edaravone significantly attenuated diabetes induced RGCs death, upregulation of ROS, ERK1/2 phosphorylation, and cleaved caspase-3 and downregulation of BDNF. These findings suggest that oxidative stress plays a pivotal role in diabetic retinal damage and that systemic administration of edaravone may slow the progression of retinal neuropathy induced by diabetes.
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影响因子：
2
作者：
Qin, Yaowu;Xu, Gezhi;Wang, Wenji
通讯作者：
Wang, Wenji
影响因子：
8.3
作者：
NISHI, H;WATANABE, T;ISHIBASHI, A
通讯作者：
ISHIBASHI, A
影响因子：
8.2
作者：
Sasaki, M.;Ozawa, Y.;Kurihara, T.;Kubota, S.;Yuki, K.;Noda, K.;Kobayashi, S.;Ishida, S.;Tsubota, K.
通讯作者：
Tsubota, K.
影响因子：
5
作者：
Imai, Shunsuke;Inokuchi, Yuta;Hara, Hideaki
通讯作者：
Hara, Hideaki
影响因子：
15.9
作者：
Barber, AJ;Lieth, E;Gardner, TW
通讯作者：
Gardner, TW