Immune activation of peripheral blood and mucosal CD3+ lymphocyte cytokine profiles in children with autism and gastrointestinal symptoms
Immune activation of peripheral blood and mucosal CD3+ lymphocyte cytokine profiles in children with autism and gastrointestinal symptoms
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DOI：
10.1016/j.jneuroim.2005.12.007
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Wakefield, AJ
中科院分区：
文献类型：
--
作者：
Ashwood, P;Wakefield, AJ
在一组自闭症谱系障碍（ASD）儿童中，已经证实了以淋巴结结节增生和小肠结肠炎为特征的胃肠道病理学。通过分析CD 3（+）淋巴细胞中的细胞内细胞因子，评估了具有胃肠道（GI）症状的ASD儿童（n = 18）和典型发展中对照（n = 27）（包括非炎症对照（NIC）和炎症GI对照克罗恩病（CD）儿童）的全身和肠粘膜免疫失调。ASD患儿外周血和粘膜中的CD 3（+）TNF α（+）和CD 3（+）IFN γ（+）均高于NIC（p < 0.004），并达到与CD相似的水平。相反，与NIC和CD对照组相比，有GI症状的ASID儿童外周和粘膜CD 3（+）IL-10（+）显著降低（p < 0.02）。此外，ASD组粘膜CD 3（+）IL-4（+）细胞较NIC组增加（p < 0.007）。在该ASID队列中，外周血和粘膜CD 3+淋巴细胞细胞内细胞因子存在独特的模式，这与显著的免疫失调一致。(c)2006 Elsevier B. V.保留所有权利。
Gastrointestinal pathology, characterized by lymphoid nodular hyperplasia and entero-colitis, has been demonstrated in a cohort of children with autistic spectrum disorder (ASD). Systemic and intestinal mucosal immune dysregulation was assessed in ASD children with gastrointestinal (GI) symptoms (n = 18), and typically developing controls (n = 27), including non-inflamed controls (NIC) and inflamed GI control children with Crohn's disease (CD), by analysis of intracellular cytokines in CD3(+) lymphocytes. In both peripheral blood and mucosa, CD3(+)TNF alpha(+) and CD3(+) IFN gamma(+) were increased in ASD children compared with NIC (p < 0.004) and reached levels similar to CD. In contrast, peripheral and mucosal CD3(+) IL-10(+) were markedly lower in ASID children with GI symptoms compared with both NIC and CD controls (p < 0.02). In addition, mucosal CD3(+) IL-4(+) cells were increased (p < 0.007) in ASD compared with NIC. There is a unique pattern of peripheral blood and mucosal CD3+ lymphocytes intracellular cytokines, which is consistent with significant immune dysregulation, in this ASID cohort. (c) 2006 Elsevier B.V. All rights reserved.