METABOLISM OF RIBAVIRIN IN RESPIRATORY SYNCYTIAL VIRUS-INFECTED AND UNINFECTED CELLS
METABOLISM OF RIBAVIRIN IN RESPIRATORY SYNCYTIAL VIRUS-INFECTED AND UNINFECTED CELLS
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DOI：
10.1128/aac.30.1.117
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发表时间：
1986-07-01
影响因子：
4.9
通讯作者：
MATTHEWS, TR
中科院分区：
文献类型：
--
作者：
SMEE, DF;MATTHEWS, TR
在未感染和呼吸道合胞病毒感染的细胞中检测利巴韦林对其单磷酸、二磷酸和三磷酸衍生物的代谢。磷酸化程度与细胞外利巴韦林浓度有关。形成的主要物种是三磷酸盐，单磷酸盐和二磷酸盐分别约占三磷酸盐的12%和4%。感染细胞中形成的三磷酸的数量是未感染细胞的2.6倍。去除药物后，三磷酸利巴韦林降解非常迅速，衰变半衰期为70至100分钟。放线菌素D抑制三磷酸的产生，并中和利巴韦林的抗病毒作用。
The metabolism of ribavirin to its mono-, di-, and triphosphate derivatives was examined in uninfected and respiratory syncytial virus-infected cells. The degree of phosphorylation was dose dependent upon extracellular ribavirin concentration. The major species formed was the triphosphate, with mono- and diphosphates being approximately 12 and 4% of the triphosphate, respectively. Amounts of triphosphate formed in infected cells were up to 2.6-fold greater than those in uninfected cells. Upon drug removal, ribavirin triphosphate degradation was very rapid, with decay half-lives of 70 to 100 min. Actinomycin D inhibited triphosphate production and also neutralized the antiviral effect of ribavirin.