Expression of stefin A is of prognostic significance in squamous cell carcinoma of the head and neck
Expression of stefin A is of prognostic significance in squamous cell carcinoma of the head and neck
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DOI：
10.1007/s00405-013-2465-5
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发表时间：
2013-11-01
影响因子：
2.6
通讯作者：
Strojan, Primoz
中科院分区：
文献类型：
--
作者：
Anicin, Aleksandar;Gale, Nina;Strojan, Primoz
溶酶体蛋白酶组织蛋白酶B和L（CB、CL）及其内源性抑制剂stefins A和B（SA、SB）与肿瘤细胞侵袭和转移相关。本研究的目的是确定 65 名可手术头颈鳞状细胞癌 (HNSCC) 患者组织切片中这些参数的免疫组织化学 (IHC) 定位，并评估观察到的 IHC 反应的预后意义。在 SCC 细胞中，CB 和 CL 的点状染色模式在核周区域尤其极化，而对于 Stefins，特征性 IHC 模式是弥漫性细胞质反应。在单变量生存分析中发现较高的 SA 免疫反应性评分具有预后优势 [局部区域控制 (LRC)，P = 0.003；无病生存（DFS），P = 0.023；疾病特异性生存率 (DSS)，P = 0.030]，并且对于在多变量环境中预测 LRC (P = 0.019) 具有显着意义。在淋巴结阳性、囊外延伸阴性的患者中，SA 阳性与良好的结局相关（LRC，P = 0.094；DFS，P = 0.013；DSS，P = 0.012）。总之，肿瘤细胞中的 SA 免疫反应性与良好的预后相关。在颈部淋巴结阳性、囊外延伸阴性的亚组中，SA 免疫反应性评分可用于识别疾病复发风险增加的患者。
Lysosomal proteases cathepsins B and L (CB, CL) and their endogenous inhibitors stefins A and B (SA, SB) are associated with tumor cell invasion and metastasis. The purpose of this study was to determine the immunohistochemical (IHC) localization of these parameters in tissue sections of 65 patients with operable head and neck squamous cell carcinoma (HNSCC) and to evaluate the prognostic significance of the observed IHC reactions. In SCC cells, a dot-like staining pattern for CB and CL was especially polarized in the perinuclear area and for stefins the characteristic IHC pattern was a diffuse cytoplasmic reaction. Higher SA immunoreactivity scores were found prognostically advantageous in univariate survival analysis [locoregional control (LRC), P = 0.003; disease-free survival (DFS), P = 0.023; disease-specific survival (DSS), P = 0.030] and appeared significant for predicting LRC (P = 0.019) in a multivariate setting. Among node-positive extracapsular extension-negative patients, SA positivity correlated with a favorable outcome (LRC, P = 0.094; DFS, P = 0.013; DSS, P = 0.012). In conclusion, SA immunoreactivity in tumor cells was related to a favorable prognosis. In the neck node-positive extracapsular extension-negative subgroup, SA immunoreactivity scores can be used to identify patients at increased risk for disease relapse.