Nuclear and cytoplasmic transfer in mammalian embryos.
Nuclear and cytoplasmic transfer in mammalian embryos.
复制标题
哺乳动物胚胎中的核和细胞质转移。
DOI：
10.1007/978-1-4613-2143-9_2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Solter,D
中科院分区：
文献类型：
--
作者：
McGrath,J;Solter,D
Manipulation of embryos (surgical and/or chemical) is an important tool in the experimental embryology of mammals. By altering the number of cells in the embryo, the relationship of cells to each other, combinations of cells from different embryos, and so forth, much has been learned about mechanisms which control normal mammalian development (for reviews, see Mintz, 1974; Gardner, 1978; Johnson, 1981). This chapter is concerned, however, only with procedures that alter the nuclear-cytoplasmic composition of the early mammalian embryo. These alterations include the introduction of foreign nuclei or cytoplasm into intact or enucleated early mammalian ova. The response of the transplanted nucleus to its new cytoplasmic environment may permit insight into the nature of nuclear-cytoplasmic communication. In addition, the ability of the transplanted nucleus to assume the role of the embryo nucleus should permit a functional assessment of nuclear potential. Similarly, the transfer of foreign cytoplasm will expose the host nucleus to cytoplasmic signals not normally encountered and thus serve to define cytoplasmic controls in development. Since experimental manipulations of this type require the technical capability to transfer nuclei or cytoplasm, a description of a nuclear-cytoplasmic transfer method devised by the authors is presented. The ability to refashion the nuclear and cytoplasmic components of the early mammalian embryo should enhance our understanding of how these two fundamental cellular compartments interact in normal development.
