Interplay between aging and unloading on oxidative stress in fast-twitch muscles.
Interplay between aging and unloading on oxidative stress in fast-twitch muscles.
复制标题
衰老和快肌氧化应激减轻之间的相互作用。
DOI：
10.1093/gerona/gls240
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Thompson,LaDoraV
中科院分区：
文献类型：
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作者：
Chen,Chiao-nanJoyce;Thompson,LaDoraV
这项研究评估了衰老对抗氧化剂适应潜力的影响，以及快肌在非负重条件下氧化损伤积累的影响。成年和老年大鼠被随机分为4组：正常负重组、后肢负重组3、7和14天。测定胫骨前肌中锰超氧化物歧化酶、铜锌超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及谷胱甘肽、羰基化蛋白和丙二醛的含量。我们发现，除铜锌超氧化物歧化酶外，大多数抗氧化剂在快肌卸载后的适应性在老年大鼠中均保持完整，但铜锌超氧化物歧化酶的活性随着卸载 14 天而下降。此外，在卸载 14 天后，丙二醛会在老年肌肉中积聚，但成人肌肉中不会积聚。总的来说，随着年龄的增长，快肌中铜锌超氧化物歧化酶的卸载适应能力会受到损害，这可能与丙二醛的积累更多有关。
This study evaluated the effect of aging on the adaptation potential of antioxidants and the accumulation of oxidative damage in fast-twitch muscles in response to non–weight-bearing conditions. Adult and old rats were randomized into 4 groups: normal weight bearing, hind-limb unloading for 3, 7, and 14 days. Activities of manganese superoxide dismutase, copper–zinc superoxide dismutase, catalase, and glutathione peroxidase and contents of glutathione, carbonylated proteins, and malondialdehyde were determined in tibialis anterior muscles. We found that the adaptability of most antioxidants in fast-twitch muscles with unloading is intact in aged rats except copper–zinc superoxide dismutase where its activity decreased with 14 days of unloading. Additionally, malondialdehyde accumulated in aged muscles with 14 days of unloading but not adult muscles. Collectively, the adaptation of copper–zinc superoxide dismutase in fast-twitch muscles with unloading is impaired with aging, which may be related to the greater accumulation of malondialdehyde.