Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture
Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture
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DOI：
10.1263/jbb.99.259
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发表时间：
2005-03-01
影响因子：
2.8
通讯作者：
Ohtake, H
中科院分区：
文献类型：
--
作者：
Konishi, M;Kishimoto, K;Ohtake, H
采用指数补料分批培养技术，研究了不同硫源对红红杜鹃（Rhodococeus erythropolis） KA2-5-1脱硫活性的影响。硫源的进料速率与乙醇的进料速率独立控制，乙醇作为碳源和能源。硫源包括二苯并噻吩（DBT）、硫酸铵、(L)-半胱氨酸、(L)-蛋氨酸和2-氨基乙磺酸。当添加DBT作为唯一硫源时，KA2-5-1细胞的最大脱硫活性约为130 mmol 2-HBP kg-cell(-1)h（-1）。类似水平的酶活性也取得了与廉价的硫酸铵通过使用指数补料分批培养技术。此外，通过增加红木KA2-5-1中DBT脱硫（dsz）操纵子和dszD基因的剂量，可以获得更高的脱硫活性。重组菌株的最大脱硫活性约为250 mmol 2-HBP kg-cell(-1)h（-1）。
The effects of sulfur sources on the desulfurization activity of Rhodococeus erythropolis KA2-5-1 were investigated by using an exponential fed-batch culture technique. The feed rate of a sulfur source was controlled independently of the feed rate of ethanol, which was used as a carbon and energy source. Among the sulfur sources examined were dibenzothiophene (DBT), ammonium sulfate, (L)-cysteine, (L)-methionine, and 2-amino-ethanesulfonic acid. When the fed-medium contained DBT as the sole sulfur source, KA2-5-1 cells showed a maximum desulfurization activity of approximately 130 mmol 2-HBP kg-cell(-1)h(-1). Similar levels of enzyme activity were also achieved with inexpensive ammonium sulfate by using the exponential fed-batch culture technique. In addition, higher levels of desulfurization activity were achieved by increasing the dosage of the DBT desulfurization (dsz) operon and dszD gene in R. erythropolis KA2-5-1. The recombinant strain showed a maximum desulfurization activity of approximately 250 mmol 2-HBP kg-cell(-1)h(-1) in the exponential fed-batch cultures.