ANALYSIS OF TN7 TRANSPOSITION
ANALYSIS OF TN7 TRANSPOSITION
复制标题
DOI：
10.1007/bf00338097
复制
发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
SHERRATT, D
中科院分区：
文献类型：
--
作者：
ROGERS, M;EKATERINAKI, N;SHERRATT, D
五个Tn7转座所需的基因产物进行了鉴定，使用遗传互补试验。其中四个（tnsA、tnsB、tnsC和tnsD）对于插入到附着位点是必需的，而第五个（tnsE）对于转座到缺乏该位点的质粒是必需的。转座至缺乏附着位点的质粒不需要tnsD。该分析使用了含有Tn7末端但不含完整Tn7基因产物的氯霉素抗性“迷你"转座子和含有克隆Tn7片段的几种相容性表达载体。转录和翻译融合的使用允许在转座子的右手端鉴定两个启动子（P1和P2），并且表明至少tnsA、tnsB和tnsC以相同的方向翻译。从P1的表达似乎被tnsB抑制。
Five gene products required for Tn7 transposition were identified using genetic complementation tests. Four of these (tnsA, tnsB, tnsC and tnsD) are essential for insertion into the attachment site, whereas the fifth (tnsE) is required for transposition to plasmids which lack this site. tnsD is not required for transposition to plasmids lacking the attachment site. This analysis used a chloramphenicol-resistant ''mini'' transposon containing Tn7 termini but no complete Tn7 gene product and several compatible expression vectors containing cloned Tn7 fragments. The used of transcriptional and translational fusions allowed the identification of two promoters (P1 and P2) at the right-hand end of the transposon and indicated that at least tnsA, tnsB and tnsC are translated in the same direction. Expression from P1 appears to be repressed by tnsB.