Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice.
Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice.
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DOI：
10.1186/s12284-014-0009-2
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发表时间：
2014-12
期刊：
影响因子：
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通讯作者：
Nishiguchi M
中科院分区：
文献类型：
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作者：
Alam MM;Nakamura H;Ichikawa H;Miyao A;Hirochika H;Kobayashi K;Yamaoka N;Nishiguchi M
天冬氨酸蛋白酶 (AP) 在植物生长、发育以及生物和非生物胁迫中发挥着重要作用。我们之前报道过水稻 AP 基因（OsAP77，Os10g0537800）的表达是由丙苯苯唑（PBZ）（一种抗病化学诱导剂）诱导的。在这项研究中，我们检查了该基因响应真菌、细菌和病毒病原体的一些特征。为了阐明OsAP77的空间和时间表达，构建了携带由OsAP77启动子驱动的编码β-葡萄糖醛酸酶（GUS）的结构基因的嵌合基因。将该构建体引入水稻中，并对转基因品系进行测试，以分析真菌、细菌和病毒感染的基因表达。接种 Magnaporthe oryzae 或 Xanthomonas oryzae pv.米曲霉揭示了每种病原体在症状部位周围的维管组织中增强的 GUS 活性。此外，接种黄瓜花叶病毒（CMV）后，GUS 活性也增加。将转基因植物浸入含有水杨酸 (SA)、异烟酸 (INA)、过氧化氢 (H2O2) 或脱落酸 (ABA) 的溶液中，仅在维管组织中表现出 GUS 活性水平的增加。 RT-PCR分析表明OsAP77不仅是由这些病原体感染诱导的，而且在用SA、INA、H2O2或ABA处理后也能诱导。接种米霉、米霉变种后插入 Tos17 的 OsAP77 敲除突变系。与野生型相比，米曲霉或巨细胞病毒表现出增强的敏感性。这些结果表明OsAP77的表达是由病原体感染和防御相关信号分子以血管组织特异性方式诱导的，并且该基因对针对真菌、细菌和病毒感染的防御反应具有积极作用。本文的在线版本 (doi:10.1186/s12284-014-0009-2) 包含补充材料，可供授权用户使用。
Aspartic protease (APs) plays important roles in plant growth, development and biotic and abiotic stresses. We previously reported that the expression of a rice AP gene (OsAP77, Os10g0537800) was induced by probenazole (PBZ), a chemical inducer of disease resistance. In this study we examined some characteristics of this gene in response to fungal, bacterial and viral pathogens. To elucidate the spatial and temporal expression of OsAP77, the chimeric gene was constructed carrying the structural gene encoding β-glucuronidase (GUS) driven by the OsAP77 promoter. This construct was introduced into rice and the transgenic lines were tested to analyze gene expression by fungal, bacterial and viral infections. Inoculation with Magnaporthe oryzae or Xanthomonas oryzae pv. oryzae revealed the enhanced GUS activities in vascular tissues surrounding the symptom sites by each pathogen. Moreover, GUS activity also increased after inoculation with Cucumber mosaic virus (CMV). Transgenic plants immersed in a solution containing salicylic acid (SA), isonicotinic acid (INA), hydrogen peroxide (H2O2) or abscisic acid (ABA) showed an increased level of GUS activity exclusively in vascular tissues. RT-PCR analysis showed that OsAP77 was induced not only by infection with these pathogens, but also after treatment with SA, INA, H2O2 or ABA. A knockout mutant line of OsAP77 by the insertion of Tos17 after inoculation with M. oryzae, X. oryzae pv. oryzae or CMV showed an enhanced susceptibility compared to wild type. These results suggest that the expression of OsAP77 is induced by pathogen infection and defense related signaling molecules in a vascular tissue specific manner and that this gene has a positive role of defense response against fungal, bacterial and viral infections. The online version of this article (doi:10.1186/s12284-014-0009-2) contains supplementary material, which is available to authorized users.