Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms
Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms
复制标题
DOI：
10.1128/aac.45.9.2475-2479.2001
复制
发表时间：
2001-09-01
影响因子：
4.9
通讯作者：
López-Ribot, JL
中科院分区：
文献类型：
--
作者：
Ramage, G;vande Walle, K;López-Ribot, JL
白色念珠菌与许多生物材料相关的感染有关。通常，这些感染与生物膜的形成有关。生物膜中的细胞表现出与自由漂浮的浮游生物细胞截然不同的表型特征，并且众所周知，它们对抗菌剂具有抗药性。因此，与生物膜有关的感染本质上很难治疗，也很难用正常的治疗方案完全根除。在这里，我们报告了一种快速和高重复性的基于微量滴度的比色分析方法，用于真菌生物膜的敏感性测试，该方法基于福尔马赞盐还原法测量固着细胞的代谢活性。用该方法对作为生物膜生长的几株白色念珠菌进行了对两性霉素B和氟康唑的体外药敏试验，证实了白色念珠菌生物膜对这些抗真菌药物的耐药性增加。由于它的简单性、与广泛使用的96孔微孔板平台的兼容性、高通量和自动化潜力，我们相信该方法是一种有前途的标准化真菌生物被膜体外抗真菌药物敏感性测试的工具。
Candida albicans is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of several C. albicans strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of C albicans biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms.