Mould extracts increase the allergic response to ovalbumin in mice
Mould extracts increase the allergic response to ovalbumin in mice
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DOI：
10.1111/j.1365-2222.2004.02076.x
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发表时间：
2004-10-01
影响因子：
6.1
通讯作者：
Hetland, G
中科院分区：
文献类型：
--
作者：
Instanes, C;Ormstad, H;Hetland, G
背景
室内空气中的霉菌暴露被认为会在易感人群中诱发哮喘。霉菌可能含有多种强效过敏原。然而，更重要的是，霉菌可能会增加对其他过敏原的过敏反应（佐剂效应）。此前，我们发现来自核盘菌细胞壁的一种β -1,3 - 葡聚糖会在小鼠模型中增加对模式过敏原卵清蛋白（OVA）的过敏反应。
目的
在本研究中，我们希望在该模型中确认来自面包酵母的另一种β -1,3 - 葡聚糖（MacroGard®，MG）的佐剂效应。更重要的是，我们希望使用与探索β - 葡聚糖作用相同的模型来探究枝孢霉（CH）和产黄青霉（PC）提取物的假定作用。
方法
将八只Balb/c小鼠为一组，分别在一只脚垫处单独注射OVA、OVA + 提取物或OVA + MG。在第21天，所有小鼠再次注射OVA，然后在第26天放血。通过酶联免疫吸附测定（ELISA）测量血清中OVA特异性IgE、IgG1和IgG2a的水平。
结果
与单独注射OVA相比，OVA + MG、OVA + CH提取物和OVA + PC提取物显著增加了OVA特异性IgE和IgG1的水平。对于所有组，抗OVA的IgG2a水平与单独注射OVA组相似。
结论
我们的结果表明，来自CH和PC的提取物以及来自面包酵母的β -1,3/1,6 - 葡聚糖对小鼠的过敏反应具有佐剂效应。
Background Exposure to moulds in indoor air is thought to induce asthma in susceptible persons. Moulds may contain several potent allergens. However, more importantly, moulds may increase the allergic response to other allergens (adjuvant effect). Previously, we have found that a beta-1,3-glucan from the cell wall of the fungus Sclerotinia sclerotiorum increases the allergic response to the model allergen ovalbumin (OVA) in a mouse model.Objective In the present study, we wanted to confirm the adjuvant effect of another beta-1,3-glucan, MacroGard(R) (MG) from baker's yeast in this model. More importantly, we wished to explore the putative effects of extracts from the moulds Cladosporium herbarum (CH) and Penicillium chrysogenum (PC) using the very same model as used to explore effects of beta-glucans.Methods Groups of eight Balb/c mice were injected with OVA alone, OVA+extract or OVA+MG, into one footpad. On day 21, all mice were reinjected with OVA, before exsanguination on day 26. The levels of OVA-specific IgE, IgG1 and IgG2a in serum were measured by ELISA.Results Compared with OVA alone, OVA+MG, OVA+CH extract and OVA+PC extract increased OVA-specific IgE and IgG1 levels significantly. For all groups, the levels of IgG2a anti-OVA remained similar to those of the OVA-alone group.Conclusions Our results show that extracts from CH and PC, and the beta-1,3/1,6-glucan from baker's yeast have adjuvant effects on the allergic response in mice.