Susceptibility of tick cell lines to infection with Alkhumra haemorrhagic fever virus.
Susceptibility of tick cell lines to infection with Alkhumra haemorrhagic fever virus.
复制标题
蜱细胞系对 Alkhumra 出血热病毒感染的易感性。
DOI：
10.1093/trstmh/trt087
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发表时间：
2013
影响因子：
2.2
通讯作者：
Ksiazek,ThomasG
中科院分区：
文献类型：
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作者：
Madani,TariqA;Abuelzein,El-TayebME;Bell-Sakyi,Lesley;Azhar,EsamI;Al-Bar,HusseinMS;Abu-Araki,Huda;Hassan,AhmedM;Masri,BadrE;Ksiazek,ThomasG
虽然Alkhumra出血热病毒（AHFV）已从蜱中分离出来，但流行病学数据表明，它是通过与动物直接接触或蚊子叮咬从牲畜传播给人类的，而不是通过蜱。方法将AHFV分别接种于硬蜱Hyalomma anatolicum（HAE/CTVM 9）、扇头蜱Rhipicephalus apturatus（RAE/CTVM 1）和软蜱Ornithodoros moubata（OME/CTVM 24）的细胞系中。接种的细胞进行直接检查，每周4周的实时逆转录PCR和IFAT使用多克隆antibodies.ResultsAHFV RNA检测在所有三个接种的蜱细胞系在整个4周的观察期内的水平高达近两倍的接种物，但他们都没有表现出细胞病变的影响。IFAT法在3种细胞株中均能检测到AHFV抗原。在LLC-MK 2细胞中培养4周后，AHFV滴度为106.650%（OME/CTVM 24）/ml和105.5TCID50/ml（RAE/CTVM 1细胞）;在HAE/CTVM 9细胞中未检测到活病毒。
BackgroundAlthough Alkhumra haemorrhagic fever virus (AHFV) has been isolated from ticks, epidemiological data suggest that it is transmitted from livestock to humans by direct contact with animals or by mosquito bites, but not by ticks. This study was carried out to assess the ability of the virus to replicate in tick cells in vitro.MethodsAHFV was inoculated into cell lines derived from the hard ticksHyalomma anatolicum(HAE/CTVM9) andRhipicephalus appendiculatus(RAE/CTVM1) and the soft tickOrnithodoros moubata(OME/CTVM24). Inoculated cells were directly examined every week for 4 weeks by real-time reverse transcription PCR and by IFAT using polyclonal antibodies.ResultsAHFV RNA was detected in all three inoculated tick cell lines throughout the 4-week observation period at levels up to almost twice that of the inoculum, but none of them exhibited a cytopathic effect. AHFV antigen could be detected in all three cell lines by IFAT. Titration of tick cell culture suspension in LLC-MK2 cells yielded AHFV titres of 106.650% tissue culture infective dose (TCID50)/ml for OME/CTVM24 and 105.5TCID50/ml for RAE/CTVM1 cells after 4 weeks of culturing; no viable virus was detected in HAE/CTVM9 cells.ConclusionThis is the first description of propagation of AHFV in tick cells.