The complete genome sequence of the Atlantic salmon paramyxovirus (ASPV)
The complete genome sequence of the Atlantic salmon paramyxovirus (ASPV)
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DOI：
10.1016/j.virol.2007.11.017
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发表时间：
2008-03-30
期刊：
影响因子：
3.7
通讯作者：
Nylund, Are
中科院分区：
文献类型：
--
作者：
Nylund, Stian;Karlsen, Marius;Nylund, Are
大西洋鲑鱼副粘病毒 (ASPV) 的完整 RNA 基因组已确定，该病毒是从患有增殖性鳃炎症 (PGI) 的大西洋鲑鱼中分离出来的。该基因组长度为 16,965 个核苷酸，由 6 个非重叠基因组成，顺序为 3'- N - P/C/V - M - F - HN - L -5'，分别编码核衣壳蛋白、磷酸蛋白、基质蛋白、融合蛋白、血凝素神经氨酸酶蛋白和大聚合酶蛋白。基因连接处含有高度保守的转录起始和终止信号序列以及与其他副粘病毒科相似的三核苷酸基因间区域。 ASPV P基因表达策略类似于呼吸道病毒和麻疹病毒，它们从初级转录本表达磷蛋白，并编辑一部分mRNA以编码辅助蛋白V和W，它还通过翻译起始的核糖体选择来编码C蛋白。氨基酸同一性的配对比较以及推导的 ASPV 蛋白序列与其他副粘病毒科同源序列的系统发育分析表明，ASPV 对呼吸道病毒属具有亲和力，但可能代表副粘病毒亚科中的一个新属。 (C) 2007 Elsevier Inc. 保留所有权利。
The complete RNA genome of the Atlantic salmon paramyxovirus (ASPV), isolated from Atlantic salmon suffering from proliferative gill inflammation (PGI), has been determined. The genome is 16,965 nucleotides in length and consists of six nonoverlapping genes in the order 3'- N - P/C/V - M - F - HN - L -5', coding for the nucleocapsid, phospho-, matrix, fusion, hemagglutinin-neuraminidase and large polymerase proteins, respectively. The gene junctions contain highly conserved transcription start and stop signal sequences and trinucleotide intergenic regions similar to those of other Paramyxoviridae. The ASPV P-gene expression strategy is like that of the respiro- and morbilliviruses, which express the phosphoprotein from the primary transcript, and edit a portion of the mRNA to encode the accessory proteins V and W, It also encodes the C-protein by ribosomal choice of translation initiation. Pairwise comparisons of amino acid identities, and phylogenetic analysis of deduced ASPV protein sequences with homologous sequences from other Paramyxoviridae, show that ASPV has an affinity for the genus Respirovirus, but may represent a new genus within the subfamily Paramyxovirinae. (C) 2007 Elsevier Inc. All rights reserved.