Shape-engineered fibroblasts: Cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy
Shape-engineered fibroblasts: Cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy
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DOI：
10.1002/jbm.a.31114
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发表时间：
2007-06-15
影响因子：
4.9
通讯作者：
Matsuda, Takehisa
中科院分区：
文献类型：
--
作者：
Kidoaki, Satoru;Matsuda, Takehisa
细胞形态调控是组织工程和生物功能材料开发中的一项重要任务，它决定了细胞在人工细胞外环境中的黏附、铺展、迁移和增殖等行为。为了加深对形状依赖性细胞力学的认识，对形状工程成纤维细胞、圆形和梭形细胞在光刻微加工表面培养的细胞弹性和肌动蛋白细胞骨架(CSK)的结构特征进行了表征，采用细胞微压痕试验和原子力显微镜(AFM)和荧光显微镜(FM)相结合的荧光观察肌动蛋白CSK。分析了细胞弹性、肌动蛋白CSK的结构特征和工程细胞形态之间的关系，并与未加工表面培养的对照细胞(称为自然延伸细胞)进行了比较。结果表明，具有稀疏或无根尖应力纤维(ASF)的梭形细胞与具有致密ASF的自然伸展的细胞具有相似的硬度。纺锤形细胞的弹性受应力纤维(SF)密度的影响较小，与自然伸展细胞的细胞弹性与SF密度呈显著正相关。这一结果表明，局部受限的黏附表面诱导的形状工程细胞，特别是高度伸长的细胞，其弹性主要受细胞形状的影响，而不是受SFS的结构特征的影响。(C)2007年威利期刊公司《生物医学杂志》81a：803-810,2007。
The regulation of cell shape, which determines cell behaviors including adhesion, spreading, migration, and proliferation in an engineered artificial extracellular milieu, is an important task in tissue engineering and in development of functional biomaterials. To deepen the understandings of shape-dependent cell mechanics, the cell elasticity and structural features of the actin cytoskeleton (CSK) were characterized for shape-engineered fibroblasts; round and spindle-shaped cells cultured on photolithographically microprocessed surfaces, employing the cellular microindentation tests and fluorescence observation of actin CSK by the combination of atomic force microscopy (AFM) and fluorescence microscopy (FM). The relationships among cell elasticity, the structural features of actin CSK, and engineered cell shape were analyzed and compared with those of control cells that had been cultured on nonprocessed surfaces (termed naturally extended cells). Results showed that the spindle-shaped cells with sparse or no apical stress fibers (ASFs) exhibited similar stiffness to that of the naturally extended cells with dense ASFs. The elasticity of spindle-shaped cells was affected only slightly by the stress fiber (SF) density, which is in marked contrast to the significant correlation shown between cell elasticity and SF density in naturally extended cells. This result implies that the elasticity of regionally restricted adhesion-surface-induced shape-engineered cells, particularly of highly elongated cells, is affected predominantly by cell shape rather than by structural features of SFs. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res 81A: 803-810, 2007.