Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
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多核苷酸的固相合成。
DOI：
10.1093/nar/10.5.1755
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发表时间：
1982
影响因子：
14.9
通讯作者：
Keiichi Itakura
中科院分区：
文献类型：
--
作者：
Hirataka Ito;Yoshimasa Ike;Satoshi Ikuta;Keiichi Itakura
利用磷酸三酯法建立了一种简单的固相合成寡脱氧核苷酸的方法。将单核苷酸肽偶联单元依次加入到具有1%二乙烯基苯的聚苯乙烯共聚物中，并以相对高产率合成了两种寡核苷酸d（CACGACCCCTCCACGT）和d（AACTGGTATTACTGGGCG）。一个循环的蒙替利肽添加约为70分钟，并且该方法特别适合于在自动合成仪可用时的合成自动化。
A simple solid phase method for the synthesis of oligodeoxyribonucleotides has been developed using the phosphotriester approach. Mononucleotide coupling units are sequentially added to the polystyrene copolymer with 1% divinylbenzene and two kinds of oligonucleotides, d(CACGACCCCTCCACGT) and d(AACTGGTATTACTGGGCG), are synthesized in a relatively high yield. One cycle of the mononucleotide addition is about 70 minutes, and this method is particularly suitable for the automation of the synthesis upon availability of an automatic synthesizer.