PEPTIDE-SYNTHESIS .2. PROCEDURES FOR SOLID-PHASE SYNTHESIS USING N-ALPHA-FLUORENYLMETHOXYCARBONYLAMINO-ACIDS ON POLYAMIDE SUPPORTS - SYNTHESIS OF SUBSTANCE-P AND OF ACYL CARRIER PROTEIN 65-74 DECAPEPTIDE
PEPTIDE-SYNTHESIS .2. PROCEDURES FOR SOLID-PHASE SYNTHESIS USING N-ALPHA-FLUORENYLMETHOXYCARBONYLAMINO-ACIDS ON POLYAMIDE SUPPORTS - SYNTHESIS OF SUBSTANCE-P AND OF ACYL CARRIER PROTEIN 65-74 DECAPEPTIDE
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DOI：
10.1039/p19810000538
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SHEPPARD, RC
中科院分区：
文献类型：
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作者：
ATHERTON, E;LOGAN, CJ;SHEPPARD, RC
使用不稳定碱的N.alpha.-9-fluorenylmethoxycarbonylamino-acids与不稳定酸的叔丁基或对烷氧基苄基侧链或羧基末端(树脂连接)保护基团结合使用，可以在异常温和的反应条件下进行固相多肽合成。避免了常规合成中所需的重复和强烈的酸性处理。描述了使用这种聚酰胺载体上的保护基团的新组合来高产率地合成十肽和十一肽酰胺(P物质)。
Use of base-labile N.alpha.-9-fluorenylmethoxycarbonylamino-acids in combination with acid-labile tert-butyl or p-alkoxybenzyl side-chain or carboxy-terminal (resin-linkage) protecting groups enables solid-phase peptide synthesis to be carried out under exceptionally mild reaction conditions. The repetitive and vigorous acidic treatments required in conventional synthesis are avoided. High-yield syntheses of a decapeptide and of an undecapeptide amide (Substance P) are described using this new combination of protecting groups on polyamide supports.