SEQUENTIAL GENE ACTIVATION BY ECDYSONE IN POLYTENE CHROMOSOMES OF DROSOPHILA-MELANOGASTER .2. EFFECTS OF INHIBITORS OF PROTEIN-SYNTHESIS
SEQUENTIAL GENE ACTIVATION BY ECDYSONE IN POLYTENE CHROMOSOMES OF DROSOPHILA-MELANOGASTER .2. EFFECTS OF INHIBITORS OF PROTEIN-SYNTHESIS
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DOI：
10.1016/s0012-1606(74)80016-3
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发表时间：
1974-01-01
影响因子：
2.7
通讯作者：
ASHBURNER, M
中科院分区：
文献类型：
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作者：
ASHBURNER, M
本文对蜕皮激素诱导的果蝇（Drosophilamelanogasteris）幼虫唾液腺染色体膨化序列进行了实验分析，并将其扩展到蛋白质合成抑制效应。抑制剂对蜕皮间喷3C和25AC的消退没有明显的影响，但减缓蜕皮激素依赖的68C的快速消退。三个早期抽吸中的两个（74EF和75B）的诱导同样不受抑制剂的影响，但其随后的消退被阻断。第三次早期抽吸（23E）的诱导受到抑制。所有五种晚期抽吸（62 E、78 D、22 C、63 E和82 F）的诱导均受到所有三种测试抗生素（放线菌酮、嘌呤霉素和茴香霉素）的抑制。这些结果的意义的想法有关的控制这个膨化序列进行了讨论。
The experimental analysis of the ecdysone-induced puffing sequence of the larval salivary gland chromosomes ofDrosophila melanogasteris extended to the effects of inhibition of protein synthesis. The inhibitors have no discernible effect on the regression of intermoult puffs 3C and 25AC but slow the ecdysone-dependent rapid regression of 68C. Induction of two of three early puffs (74EF and 75B) is similarly unaffected by inhibitors, but their subsequent regression is blocked. Induction of a third early puff (23E) is inhibited. Induction of all five late puffs (62E, 78D, 22C, 63E, and 82F) is inhibited by all three antibiotics tested (cycloheximide, puromycin, and anisomycin). The significance of these results for ideas concerning the control of this puffing sequence is discussed.