Isolation and characterization of cDNA clones expressed under male sex expression conditions in a monoecious cucumber plant (Cucumis sativus L. cv. Winter Long)
Isolation and characterization of cDNA clones expressed under male sex expression conditions in a monoecious cucumber plant (Cucumis sativus L. cv. Winter Long)
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DOI：
10.1007/s10681-005-9023-1
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发表时间：
2005-12-01
期刊：
影响因子：
1.9
通讯作者：
Hur, Y
中科院分区：
文献类型：
--
作者：
Cho, JK;Koo, DH;Hur, Y
为了克隆性别决定基因，差异筛选的cDNA文库和差异显示反转录PCR（DDRT-PCR）进行，从韩国黄瓜品种，表现出不同的性别表达反应，不同的光周期的顶端组织。通过对cDNA文库的差异筛选，克隆了两个仅在特定雄性表达条件下表达的基因（CsM 1和CsM 2），并命名为CsCYR（Cucumis sativus cyclin-related）和CsCYR（Cucumis sativus cyclin-related）。sativus cyclophilin）。这两个基因似乎都是单拷贝基因，并且都在雄性表达SD（短日照）条件下表达。CsM_（10）C.通过DDRT-PCR分离出大蒜雄性特异性克隆10），并在雄性表达条件下优先表达。CsM 10基因编码一个非编码RNA，并含有一个179 bp的保守序列，在三个基因中发现，已被归类为生物胁迫信号相关的非翻译RNA（或非编码RNA）组。CsM 10基因也是一个单拷贝基因，定位于6号染色体端粒区，在不同组织、发育阶段和光周期中表达不同。基于获得的序列结构和表达模式的CsM 10及其同系物，我们建议，CsM 10的功能似乎发生在RNA水平。
In order to clone sex determination genes, both differential screening of a cDNA library and differential display reverse transcription PCR (DDRT-PCR) were conducted, using apex tissues from a Korean cucumber cultivar that exhibited different sex expression responses to differing photoperiods. Two genes (CsM1 and CsM2) expressed only under specific male expression conditions were then cloned via differential screening of a cDNA library and designated CsCYR (Cucumis sativus cyclin-related) and CsCYP (C. sativus cyclophilin). Both genes appear to be single-copy genes, and both express under male-expressing SD (short-day) conditions. CsM10 (C. sativus male specific clone 10) was isolated via DDRT-PCR, and was expressed preferentially under male expression conditions. The CsM10 gene encodes a non-coding RNA, and harbors a 179bp-conserved sequence, found in three genes which have been classified as a biotic stress signal-associated non-translatable RNA (or non-coding RNA) group. The CsM10 gene is also a single-copy gene, which is localized in the telomeric region of chromosome 6, and its expression is different in different tissues, developmental stages, and photoperiods. Based on the obtained sequence structures and expression patterns of CsM10 and its homologues, we suggest that CsM10 function appears to occur at the RNA level.