Histopathology as a tool for the evaluation of endocrine disruption in zebrafish (Danio rerio)
Histopathology as a tool for the evaluation of endocrine disruption in zebrafish (Danio rerio)
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组织病理学作为评估斑马鱼（Danio rerio）内分泌干扰的工具
DOI：
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发表时间：
2003
影响因子：
4.1
通讯作者：
J. Vos
中科院分区：
文献类型：
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作者：
L. V. D. van der Ven;P. Wester;J. Vos
组织学作为评估鱼类内分泌干扰的工具的重要性取决于适当终点的选择和解释，正如对雌激素 17β-雌二醇 (E2) 和不可芳香化雄激素 17-甲基二氢睾酮 (MDHT) 暴露影响的分析所说明的那样。 E2导致卵巢中卵黄卵母细胞消失，睾丸中相对较大的嗜酸性细胞面积增加，在高倍放大下被鉴定为精原细胞；正如组织形态计量学所示，这是相对增加，因为生精囊肿尺寸减小，精母细胞囊肿相对减少。 E2 还诱导血管和间质中嗜酸性液体的积累，经免疫组织化学证实为卵黄蛋白原，肝脏中的嗜碱性也与卵黄蛋白原的产生有关。 MDHT 诱导睾丸中支持细胞的活化，并减少卵巢中卵黄发生前卵母细胞的存在和卵黄发生前卵母细胞的生长。这些观察表明在全身切片上检查多个器官系统以及应用足够的放大倍率的优势。纳入形态测定和免疫组织化学等其他技术对于进一步揭示内分泌干扰物的潜在影响很有价值。
The importance of histology as a tool in the evaluation of endocrine disruption in fish depends on the choice and interpretation of appropriate endpoints, as is illustrated by the analysis of the effects of exposure to the estrogen 17β‐estradiol (E2) and the nonaromatizable androgen 17‐methyldihydrotestosterone (MDHT). The E2 led to the disappearance of vitellogenic oocytes in the ovary and an increased area of relatively large, eosinophilic cells in the testis, which were identified as spermatogonia under high‐power magnification; this was a relative increase, as was shown by histomorphometry, because of a decreased size of spermatogenic cysts and a relative decrease of spermatocyte cysts. The E2 also induced an accumulation of acidophilic fluid in vessels and interstitial spaces, confirmed by immunohistochemistry as vitellogenin, and basophilia in the liver also associated with the production of vitellogenin. The MDHT induced activation of Sertoli cells in the testis and a decreased presence of vitellogenic oocytes and a reduced growth of previtellogenic oocytes in the ovary. These observations indicate the advantages of examining multiple organ systems on whole‐body sections and the application of adequate magnifications. Inclusion of additional techniques such as morphometry and immunohistochemistry is valuable to further uncover insidious effects of endocrine disruptors.
影响因子：
3.6
作者：
T. Sperry;Peter Thomas
通讯作者：
T. Sperry;Peter Thomas