Modulation of HLA antigens in response to the binding of epidermal growth factor by A431 cells.
Modulation of HLA antigens in response to the binding of epidermal growth factor by A431 cells.
复制标题
A431 细胞响应表皮生长因子的结合而调节 HLA 抗原。
DOI：
10.1016/0014-5793(88)80852-4
复制
发表时间：
1988
期刊：
影响因子：
3.5
通讯作者：
Edidin,M
中科院分区：
文献类型：
--
作者：
Hosoi,K;Kittur,DS;Edidin,M
在之前的一项研究中 [(1984) J. Cell Biol。 [98, 725–731]我们发现，A431 癌细胞上的人类 MHC、HLA 抗原水平在这些细胞结合表皮生长因子 (EGF) 后降低。在这里，我们使用流式细胞术来确定不同剂量和时间的EGF治疗对HLA表达的影响。然后我们表明，HLA 表达的减少与免疫沉淀表面 HLA 抗原磷酸化水平的降低相关，尽管细胞与 EGF 的较长时间接触增加了表面 HLA 表达及其磷酸化水平。 EGF 处理细胞中 HLA 抗原的横向扩散低于对照细胞。测得的较低扩散系数可能与 HLA 抗原磷酸化降低有因果关系。
In a previous study [(1984) J. Cell Biol. 98, 725–731] we showed that the level of human MHC, HLA antigens on A431 carcinoma cells is reduced after these cells bind epidermal growth factor (EGF). Here we use flow cytometry to determine the effects of various doses and times of EGF treatment on HLA expression. We then show that the reduction in HLA expression is associated with a reduction in the level of phosphorylation of immunoprecipitable surface HLA antigens, although longer exposure of cells with EGF increased both surface HLA expression and their phosphorylation levels. Lateral diffusion of HLA antigens is lower in EGF-treated than in control cells. The lower diffusion coefficients measured may be causally related to the decreased phosphorylation of HLA antigens.