Bacteroides graminisolvens sp nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste
Bacteroides graminisolvens sp nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste
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DOI：
10.1099/ijs.0.008268-0
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Ueki, Katsuji
中科院分区：
文献类型：
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作者：
Nishiyama, Tomomi;Ueki, Atsuko;Ueki, Katsuji
从处理牛场废弃物的产甲烷反应器的植物残渣中分离到一株严格厌氧的细菌，命名为XDT-1（T）。该菌株的细胞为革兰氏阴性、不运动、不形成孢子的杆状细胞。生长需要血红素。该菌株利用木聚糖以及各种糖，包括阿拉伯糖、木糖、葡萄糖、甘露糖、纤维二糖、棉子糖、淀粉和果胶。该菌株产生乙酸，丙酸和琥珀酸盐的存在下，从血红素。生长的最适pH为约7.2，最适生长温度为30-35 ℃。该菌株对胆汁敏感。该菌细胞脂肪酸主要为anteiso-C-15：0和iso-C-17：0 3-OH，呼吸醌主要为MIK-10（H-0），基因组DNA G+C含量为38.0mol%。基于16 S rRNA基因序列的系统发育分析将该菌株置于拟杆菌门。XDT-1（T）与卵形拟杆菌（Bacteroides ovatus）NCTC 11153（T）的16 S rRNA基因序列相似性为94.2%。根据系统发育、生理生化和化学分类学分析，菌株XDT-1（T）代表拟杆菌属的一个新种，命名为Bacteroides graminisolvens sp. nov.。模式菌株为XDT-1（T）（=JCM 15093（T）=DSM 19988（T））。
A strictly anaerobic bacterial strain, designated XDT-1(T), was isolated from plant residue from a methanogenic reactor treating waste from cattle farms. Cells of the strain were Gram-negative, non-motile, non-spore-forming rods. Haemin was required for growth. The strain utilized xylan as well as various sugars including arabinose, xylose, glucose, mannose, cellobiose, raffinose, starch and pectin. The strain produced acetate, propionate and succinate from saccharides in the presence of haemin. The optimum pH for growth was approximately 7.2 and the optimum growth temperature was 30-35 degrees C. The strain was sensitive to bile. The major cellular fatty acids of the strain were anteiso-C-15:0 and iso-C-17:0 3-OH, MIK-10(H-0) was the major respiratory quinone and the genomic DNA G+C content was 38.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the strain in the phylum Bacteroidetes. The closest phylogenetic neighbour of strain XDT-1(T) was Bacteroides ovatus NCTC 11153(T), with a 16S rRNA gene sequence similarity of 94.2%. On the basis of data from the phylogenetic, physiological and chemotaxonomic analyses, strain XDT-1(T) represents a novel species of the genus Bacteroides, for which the name Bacteroides graminisolvens sp. nov. is proposed. The type strain is XDT-1(T) (=JCM 15093(T) =DSM 19988(T)).