Human CD1-restricted T cell recognition of lipids from pollens.
Human CD1-restricted T cell recognition of lipids from pollens.
复制标题
人类CD1限制的T细胞对脂质的脂质识别。
DOI：
10.1084/jem.20050773
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发表时间：
2005-07-18
影响因子：
15.3
通讯作者：
Spinozzi, Fabrizio
中科院分区：
文献类型：
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作者：
Agea, Elisabetta;Russano, Anna;Bistoni, Onelia;Mannucci, Roberta;Nicoletti, Ildo;Corazzi, Lanfranco;Postle, Anthony D;De Libero, Gennaro;Porcelli, Steven A;Spinozzi, Fabrizio
植物花粉是刺激过敏反应的环境抗原的重要来源。除了作为外来蛋白质抗原的载体外，它们还含有含有饱和和不饱和脂肪酸的脂类，这是高等植物繁殖所必需的。CD1家族是一类非多态的主要组织相容性复合体相关分子，在哺乳动物中高度保守，已被证明可向T细胞提供微生物和自身脂质。在这里，我们提供了花粉脂类可能通过CD1依赖的途径被人类T细胞识别为抗原的证据。在从柏树籽粒中提取的磷脂中，磷脂酰胆碱和磷脂酰乙醇胺能够刺激柏树敏感者T细胞的增殖。磷脂的识别涉及多种细胞类型，主要是CD_4~+T细胞抗原受体αβ~+，一些CD_4~+−、CD_8~+、−~γδ~+，少数V_(α)24I~+自然杀伤-T细胞，需要CD1a~+和CD1d~+的抗原提呈细胞。应答T细胞分泌IL-4和干扰素-γ，部分病例分泌IL-10和转化生长因子-β，为免疫球蛋白E的产生提供帮助。对花粉磷脂的反应在授粉季节从过敏受试者的血液样本中最为明显，在接触结核分枝杆菌的医护人员中普遍缺乏，但在非过敏受试者中偶尔出现。最后，过敏者，但不是正常受试者，表现出循环特异性IgE以及对磷脂的皮肤红肿和红肿反应。
Plant pollens are an important source of environmental antigens that stimulate allergic responses. In addition to acting as vehicles for foreign protein antigens, they contain lipids that incorporate saturated and unsaturated fatty acids, which are necessary in the reproduction of higher plants. The CD1 family of nonpolymorphic major histocompatibility complex–related molecules is highly conserved in mammals, and has been shown to present microbial and self lipids to T cells. Here, we provide evidence that pollen lipids may be recognized as antigens by human T cells through a CD1-dependent pathway. Among phospholipids extracted from cypress grains, phosphatidyl-choline and phosphatidyl-ethanolamine were able to stimulate the proliferation of T cells from cypress-sensitive subjects. Recognition of phospholipids involved multiple cell types, mostly CD4+ T cell receptor for antigen (TCR)αβ+, some CD4−CD8− TCRγδ+, but rarely Vα24i + natural killer–T cells, and required CD1a+ and CD1d+ antigen presenting cell. The responding T cells secreted both interleukin (IL)-4 and interferon-γ, in some cases IL-10 and transforming growth factor-β, and could provide help for immunoglobulin E (IgE) production. Responses to pollen phospholipids were maximally evident in blood samples obtained from allergic subjects during pollinating season, uniformly absent in Mycobacterium tuberculosis–exposed health care workers, but occasionally seen in nonallergic subjects. Finally, allergic, but not normal subjects, displayed circulating specific IgE and cutaneous weal and flare reactions to phospholipids.