Determination of fluorescein and fluorescein monoglucuronide excreted in urine.
Determination of fluorescein and fluorescein monoglucuronide excreted in urine.
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尿中排泄的荧光素和荧光素单葡萄糖苷酸的测定。
DOI：
10.1248/cpb.28.2812
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发表时间：
1980
影响因子：
1.7
通讯作者：
Z. Tamura
中科院分区：
文献类型：
--
作者：
S. C. Chen;H. Nakamura;Z. Tamura
建立了尿中荧光素和单lucuronide荧光素的简单荧光测定方法。通过测定游离荧光素在494 nm激发和516 nm发射下的天然荧光来测定游离荧光素。用β-葡萄糖醛酸酶在37°温度下水解20分钟后测定其偶联物为荧光素。采用该方法研究肝损伤Wistar大鼠与正常大鼠的糖醛酸化功能。
A simple method for the fluorometric determination of fluorescein and fluorescein monoglucuronide in urine was developed. Free fluorescein was determined by measuring its native fluorescence at 494 nm excitation and 516 nm emission. The conjugate was determined as fluorescein after hydrolysis at 37°for 20 min with β-glucuronidase. This method was applied to investigate the glucuronidation function of Wistar rats with liver injury in comparison with that of normal rats.