Morphology and function of canine small intestinal autografts: with particular interest in the influence of ex vivo graft irradiation.
Morphology and function of canine small intestinal autografts: with particular interest in the influence of ex vivo graft irradiation.
复制标题
犬小肠自体移植物的形态和功能：特别关注离体移植物辐射的影响。
DOI：
10.1016/s1091-255x(03)00033-7
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Murase,Noriko
中科院分区：
文献类型：
--
作者：
Ishikawa,Takashi;Iwanami,Kotaro;Okuda,Toyokazu;Zhu,Yue;Fukuda,Akinari;Zhang,Shimin;Ou,Junhai;Nalesnik,MichaelA;Venkataramanan,Raman;Murase,Noriko
肠移植过程使移植肠早期功能缺陷。移植物照射已被用于调节移植后免疫反应;然而，辐射可能导致移植肠功能的进一步恶化。使用犬模型，我们研究了移植过程的影响，有和没有离体移植物辐射，对早期肠移植物功能和组织病理学的影响用（n= 8）或不用（n= 5）7 - 5戈伊离体移植物照射进行自体肠移植的远亲猎犬，粘液细胞色素P450和P-糖蛋白，常规免疫组织病理学，和肠吸收功能进行了研究。手术后体重增加缓慢，但照射组和非照射组相当。在移植后早期，两组均显示肠吸收缺陷，与细胞色素P450 3A 4活性降低和P-糖蛋白表达降低有关，移植后28天，两组均恢复正常。组织学上，移植后1小时，上皮细胞凋亡率略有增加;然而，没有证据表明存在组织病理学异常，包括与辐射相关的动脉变化。此外，固有层中T和B淋巴细胞的频率不受移植手术或离体照射的显著影响。因此，移植后早期，肠功能受损，口服免疫抑制剂的有效性显著改变。移植物照射未诱导肠功能进一步缺陷或引起组织病理学改变。异常
The intestinal transplantation procedure obligates an early functional deficit in intestinal grafts Graft irradiation has been used to modulate post-transplant immune reactions; however, irradiation may cause further deterioration of the function of the transplanted intestine Using the canine model, we investigated the influence of the transplant procedures, with and without ex vivo graft irradiation, on early intestinal graft function and histopathology Outbred hound dogs underwent autointestinal transplantation with (n= 8) or without (n= 5) 7 5 Gy ex vivo graft irradiation Mucosal cytochrome P450 and P-glycoprotein, routine immunohistopathology, and intestinal absorptive function were studied Weight gain was slow after surgery, but was comparable in the irradiated and nonirradiated groups During the early posttransplant period, both groups showed defects in intestinal absorption, associated with decreased cytochrome P450 3A4 activity and reduced P-glycoprotein expression, regardless of graft irradiation These changes returned to normal in both groups by day 28 Histopathologically, epithelial apoptosis showed a slight increase 1 hour after transplantation; however, there was no evidence of histopathologic abnormalities including arterial changes associated with irradiation In addition, the frequency of T and B lymphocytes in the lamina propria were not significantly influenced by the transplant surgery or ex vivo irradiation Thus, early after transplantation, intestinal function was impaired and effectiveness of orally administered immunosuppressive drugs was significantly altered Graft irradiation did not induce further defects in intestinal function or cause histopathologic abnormalities.