Jasmonate Signaling Enhances RNA Silencing and Antiviral Defense in Rice
Jasmonate Signaling Enhances RNA Silencing and Antiviral Defense in Rice
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茉莉酸信号传导增强水稻的 RNA 沉默和抗病毒防御
DOI：
10.1016/j.chom.2020.05.001
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发表时间：
2020-07-08
影响因子：
30.3
通讯作者：
Li, Yi
中科院分区：
文献类型：
--
作者：
Yang, Zhirui;Huang, Yu;Li, Yi
小分子RNA介导的RNA沉默是高等植物抗病毒的重要机制。已显示RNA沉默组分可通过病毒感染上调。然而，上调的潜在机制在很大程度上仍然未知。在这里，我们发现茉莉酸（JA）信号转导转录激活Argonaute 18（AGO18），这是一种核心RNA沉默组分，通过螯合miR168和miR528促进水稻抗病毒防御，这抑制了关键的抗病毒防御蛋白。在机制上，JA应答性转录因子JAMYB直接结合AGO18启动子以激活AGO18转录。水稻条纹病毒（RSV）外壳蛋白（CP）触发JA积累并上调JAMYB启动这一宿主防御网络。我们的研究揭示了JA信号通路和抗病毒RNA沉默通路之间存在调控串扰，并阐明了CP介导的单子叶作物病毒抗性的分子机制。
Small RNA-mediated RNA silencing is an important antiviral mechanism in higher plants. It has been shown that RNA silencing components can be upregulated by viral infection. However, the mechanisms underlying the upregulation remain largely unknown. Here, we show that jasmonate (JA) signaling transcriptionally activates Argonaute 18 (AGO18), a core RNA silencing component that promotes rice antiviral defense through sequestering miR168 and miR528, which repress key antiviral defense proteins. Mechanistically, the JA-responsive transcription factor JAMYB directly binds to the AGO18 promoter to activate AGO18 transcription. Rice stripe virus (RSV) coat protein (CP) triggers JA accumulation and upregulates JAMYB to initiate this host defense network. Our study reveals that regulatory crosstalk exists between the JA signaling and antiviral RNA silencing pathways and elucidates a molecular mechanism for CP-mediated viral resistance in monocot crops.