Remodeling of the corpora cardiaca and the corpora allata during adult metamorphosis in Bombyx mori: identification of invisible corpora cardiaca by the expression of adipokinetic hormone
Remodeling of the corpora cardiaca and the corpora allata during adult metamorphosis in Bombyx mori: identification of invisible corpora cardiaca by the expression of adipokinetic hormone
复制标题
DOI：
10.1007/s13355-010-0013-y
复制
发表时间：
2011-02-01
影响因子：
1.3
通讯作者：
Hiruma, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kaneko, Yu;Shinoda, Tetsuro;Hiruma, Kiyoshi
鉴定家蚕蛹期和成虫期的心体（CC）是有问题的，因为在相关的异形体（CA）附近存在外观相似的组织。为了寻找CC，我们使用脂肪动力激素II （AKH-II）基因作为探针进行原位杂交实验，因为AKH-II只在CC中表达，化蛹后不久，CC和CA开始向食管移动，并在4天内紧密附着在食管上；在此期间，CC和CA之间的距离从65 μ m增加到507 μ m。在重塑期间，CC变平，尽管CA保持球形直到成体蜕皮。原位杂交分析表明，在成虫发育过程中正确鉴定出CC后，参与JH合成的所有甲羟酸酶和幼虫激素酸o -甲基转移酶的mRNA表达仅发生在CA细胞中，这表明CC-CA复合物中酶mRNA数量的波动仅来自CA细胞（Kinjoh等人在Insect Biochem Mol Biol 37:808-818, 2007）。
It is problematic to identify the corpora cardiaca (CC) of pupal and adult stages of Bombyx mori because of the presence of tissues similar in appearance near the associated corpora allata (CA). To find the CC, we performed in situ hybridization experiments using the adipokinetic hormone II (AKH-II) gene as a probe, as AKH-II expression occurs only in the CC. Shortly after pupation, the CC and CA began to move toward the esophagus and had attached there tightly by 4 days; during this period, the distance between the CC and CA increased from 65 to 507 mu m. The CC became flattened during the remodeling period, although the CA stayed globular until adult ecdysis. In situ hybridization analyses, after the CC had been correctly identified during adult development, showed that the mRNA expression of all the mevalonate enzymes and juvenile hormone acid O-methyltransferase involved in JH synthesis occurred only in CA cells, indicating that the fluctuations of enzyme mRNA amounts in CC-CA complexes (Kinjoh et al. in Insect Biochem Mol Biol 37:808-818, 2007) were derived solely from those in the CA.