Desulfovibrio gilichinskyi sp. nov., a cold-adapted sulfate-reducing bacterium from a Yamal Peninsula cryopeg.
Desulfovibrio gilichinskyi sp. nov., a cold-adapted sulfate-reducing bacterium from a Yamal Peninsula cryopeg.
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吉利钦斯基脱硫弧菌 sp.
DOI：
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发表时间：
2019
影响因子：
2.8
通讯作者：
V. Shcherbakova
中科院分区：
文献类型：
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作者：
Yana Ryzhmanova;T. Abashina;D. Petrova;V. Shcherbakova
从亚马尔半岛永久冻土层中分离出一种耐冷、不形成孢子的硫酸盐还原细菌，菌株 K3ST。菌株 K3ST 在零下温度下生长，并且需要 Na+ 才能生长。新细菌能够在硫酸盐存在下使用乳酸、甲酸、丙酮酸、富马酸、丙氨酸、乙醇和分子氢作为电子供体，并在乳酸存在下使用硫酸盐、亚硫酸盐、硫代硫酸盐和元素硫作为电子受体。 Fe(III)-柠檬酸盐和 Fe(III)-EDTA 被还原，但没有明显的生长。主要极性脂质为磷脂酰丝氨酸、磷脂酰乙醇胺、磷脂、心磷脂和氨基脂质；主要细胞脂肪酸为C16 : 1ω7、C16 : 0和C18 : 1ω7；主要的类异戊二烯醌是 MK-6 (H2)。发现基因组DNA G+C 含量为42.33 mol%。系统发育分析表明，该新分离株的最近亲是Desulfovibrio ferrireducens菌株61T，其16S rRNA基因相似性为97.1%。此外，菌株K3ST和D.ferrireducens 61T之间的ANI值为82.1 %。根据菌株K3ST的基因组和多相分类数据，我们得出结论，该菌株是新种Desulfovibrio gilichinskyi sp的代表。十一月（=VKM B-2877T=DSM 100341T）。
A psychrotolerant non-spore-forming sulfate-reducing bacterium, strain K3ST, was isolated from a Yamal Peninsula cryopeg within permafrost. Strain K3ST grew at subzero temperatures and required Na+ for growth. The new bacterium was able to use lactate, formate, pyruvate, fumarate, alanine, ethanol and molecular hydrogen as electron donors in the presence of sulfate, and used sulfate, sulfite, thiosulfate and elemental sulfur as electron acceptors in the presence of lactate. Fe(III)-citrate and Fe(III)-EDTA were reduced without visible growth. Major polar lipids were рhosphatidylserine, рhosphatidylethanolamine, phospholipids, cardiolipin and aminolipid; major cellular fatty acids were C16 : 1ω7, C16 : 0 and C18 : 1ω7; and the predominant isoprenoid quinone was MK-6 (H2). The genomic DNA G+C content was found to be 42.33 mol%. Phylogenetic analysis showed that the closest relative of the new isolate was Desulfovibrio ferrireducens strain 61T with 97.1 % 16S rRNA gene similarity. In addition, the ANI value between strain K3ST and D. ferrireducens 61T was 82.1 %. On the basis of the genomic and polyphasic taxonomy data of strain K3ST, we conclude that the strain is a representative of a novel species Desulfovibrio gilichinskyi sp. nov. (=VKM B-2877T=DSM 100341T).