Diversity of the promoting action of cyclosporine on the induction of murine lymphoid tumors.
Diversity of the promoting action of cyclosporine on the induction of murine lymphoid tumors.
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环孢素对小鼠淋巴肿瘤诱导作用的多样性。
DOI：
10.1093/carcin/9.6.1091
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Shinozuka,H
中科院分区：
文献类型：
--
作者：
Hattori,A;Kunz,HW;Gill3rd,TJ;Pan,SF;Shinozuka,H
我们以前已经证明，环孢菌素（CsA），一个强大的免疫抑制剂，促进发展的胸腺淋巴瘤在瑞士韦伯斯特小鼠开始与一个单一的亚致癌剂量的N-甲基-N-亚硝基脲和促进自发发展的胸腺肿瘤在AKR小鼠。在本研究中，我们研究了用单剂量γ辐射启动小鼠是否改变了CsA促进淋巴瘤诱导的靶细胞特异性。将雄性Swas韦伯斯特和C57 B/6小鼠分成四组。第1组和第2组小鼠接受单次γ射线照射（350 rad），10天后第1组和第2组分别给予基础饲料（含0.015%CsA）25-35周。第3组和第4组由未接受辐射的对照小鼠组成，并维持基础或CsA饮食。第1、3和4组中任一品系的小鼠均未发生肿瘤。39只瑞士韦伯斯特小鼠中有18只（46%）接受放射治疗，随后给予CsA饮食，发生了累及肠系膜淋巴结和脾脏的肿瘤。肿瘤细胞表面有免疫球蛋白，Thy 1阴性，提示B细胞系淋巴瘤。4只小鼠（10%）发生胸腺肿瘤，Thy 1阳性。14例C57 B/6经放射线和CsA处理后，7例（50%）发生胸腺肿瘤，而无一例发生B细胞系肿瘤。结果表明，CsA是诱导小鼠淋巴瘤的有效促进剂，并且细胞类型由引发剂的类型和小鼠品系决定。
We have previously demonstrated that cylosporine (CsA), a powerful immunosuppressant, enhanced the development of thymic lymphomas in Swiss Webster mice initiated with a single subcarcinogenic dose ofN-methyi-N-nitrosourea and enhanced the spontaneous development of thymic tumors in AKR mice. In the present study, we examined whether the initiation of mice with a single dose of γ-radiation modified the target cell specificity of the CsA promotion of lymphoma induction. Male Swas Webster and C57B/6 mice were divided into four groups. The mice in group 1 and 2 of both strains were given a single dose (350 rad) of -γ-radiation; 10 days thereafter group 1 was given a basal diet and group 2 a basal diet containing 0.015% CsA for 25–35 weeks. Groups 3 and 4 consisted of control mice without radiation and were maintained on a basal or a CsA diet. None of the mice in either strain in groups 1, 3 and 4 developed tumors. Eighteen out of 39 (46%) Swiss Webster mice that received radiation followed by a CsA diet developed tumors involving mesenteric lymph nodes and the spleen. The tumor cells had immunoglobulins on their surface and were negative for Thy 1, suggesting lymphomas of B cell lineage. Four mice (10%) developed thymic tumors which were positive for Thy 1. Seven of 14 (50%) C57B/6 treated with radiation and CsA developed thymic tumors, while none developed tumors of B cell lineage. The results indicate that CsA is a potent promoter of the induction of lymphomas in mice and that the cell type is determined by the type of initiating agents and the strain of mice.