Identification of nonvisual photomotor response cells in the vertebrate hindbrain.
Identification of nonvisual photomotor response cells in the vertebrate hindbrain.
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DOI：
10.1523/jneurosci.3689-12.2013
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发表时间：
2013-02-27
期刊：
影响因子：
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通讯作者：
Peterson RT
中科院分区：
文献类型：
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作者：
Kokel D;Dunn TW;Ahrens MB;Alshut R;Cheung CY;Saint-Amant L;Bruni G;Mateus R;van Ham TJ;Shiraki T;Fukada Y;Kojima D;Yeh JR;Mikut R;von Lintig J;Engert F;Peterson RT
非视觉感光使动物能够在没有视觉的情况下感知光线。然而，非视觉光行为的细胞和分子机制知之甚少，特别是在脊椎动物。在这里，我们描述了光运动反应（PMR），一个强大的和可重复的一系列运动行为在斑马鱼引起的视觉波长的光，但不需要眼睛，松果体或其他典型的脑深部光感受器官。不像典型的非视觉通路的相对缓慢的影响，运动回路强烈和快速（秒）招募期间PMR行为。我们发现，后脑既必要又足够驱动这些行为。使用体内钙成像，我们确定了一个离散的神经元在后脑内的反应，光镜PMR行为。视觉周期的药理学抑制阻断PMR行为，表明视蛋白为基础的光感受器控制这种行为。这些数据代表了脊椎动物后脑中第一个已知的感光电路。
Non-visual photosensation enables animals to sense light without sight. However, the cellular and molecular mechanisms of non-visual photobehaviors are poorly understood, especially in vertebrate animals. Here, we describe the photomotor response (PMR), a robust and reproducible series of motor behaviors in zebrafish that is elicited by visual wavelengths of light, but does not require the eyes, pineal gland or other canonical deep-brain photoreceptive organs. Unlike the relatively slow effects of canonical non-visual pathways, motor circuits are strongly and quickly (seconds) recruited during the PMR behavior. We find that the hindbrain is both necessary and sufficient to drive these behaviors. Using in vivo calcium imaging, we identify a discrete set of neurons within the hindbrain whose responses to light mirror the PMR behavior. Pharmacological inhibition of the visual cycle blocks PMR behaviors, suggesting that opsin-based photoreceptors control this behavior. These data represent the first known light-sensing circuit in the vertebrate hindbrain.