Phagocytosis of Salmonella montevideo by human neutrophils: immune adherence increases phagocytosis, whereas the bacterial surface determines the route of intracellular processing.
Phagocytosis of Salmonella montevideo by human neutrophils: immune adherence increases phagocytosis, whereas the bacterial surface determines the route of intracellular processing.
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人中性粒细胞对蒙得维的亚沙门氏菌的吞噬作用：免疫粘附增加吞噬作用，而细菌表面决定细胞内处理的途径。
DOI：
10.1086/430947
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ghiran,Ionita
中科院分区：
文献类型：
--
作者：
Pilsczek,FlorianH;Nicholson-Weller,Anne;Ghiran,Ionita
补体调理的颗粒成为免疫粘附到人红细胞上的补体受体1（CR1或CD35），允许颗粒被肝脏和脾脏中的吞噬细胞摄取。我们研究了免疫粘附在人中性粒细胞摄取和杀死蒙得维的亚沙门氏菌中的作用。暴露于血清诱导鞭毛的损失和促进免疫粘附，这是其次是更有效的吞噬和杀伤，与暴露后的血清调理，游离细菌。细菌杀伤的一个相关因素是吞噬体与溶酶体的融合，这可以通过LysoTracker或溶酶体相关膜蛋白2与细胞内细菌的共定位来监测。在5分钟时，免疫粘附细菌的吞噬融合明显快于非免疫粘附细菌，但到35分钟时，两组之间的差异很小。免疫粘附也促进了抗体补体调理荧光微球的摄入，但与细菌不同，大多数内化的微球未能与溶酶体融合。然而，加入脂多糖，Toll样受体配体，微球指导其细胞内运输，导致快速溶酶体融合。因此，免疫粘附促进吞噬作用，但细胞内处理的途径取决于靶的分子性质，并且不依赖于宿主补体和抗体
Complement-opsonized particles become immune adherent to complement receptor 1 (CR1 or CD35) on human erythrocytes, allowing particles to be ingested by phagocytes in the liver and the spleen. We investigated the role that immune adherence plays in the uptake and killing ofSalmonella montevideoby human neutrophils. Exposure to serum induced loss of flagella and facilitated immune adherence, which was followed by more-efficient phagocytosis and killing, compared with that after exposure to serum-opsonized, free bacteria. One correlate of bacterial killing is the fusion of phagosomes with lysosomes, which can be monitored by LysoTracker or lysosomal-associated membrane protein 2 colocalization with the intracellular bacteria. At 5 min, phagolysosmal fusion was significantly faster for immune-adherent bacteria than for non–immune-adherent bacteria, but, by 35 min, the difference between the 2 groups was minimal. Immune adherence also facilitated the ingestion of antibody complement–opsonized fluorescent microspheres, but, unlike bacteria, most internalized microspheres failed to fuse with lysosomes. However, addition of lipopolysaccharide, a Toll-like receptor ligand, to microspheres directed their intracellular trafficking, resulting in rapid lysosomal fusion. Thus, immune adherence facilitates phagocytosis, but the route of intracellular processing depends on the molecular nature of the target and is independent of host complement and antibody