CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.
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DOI：
10.1093/nar/gkm360
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发表时间：
2007-07
影响因子：
14.9
通讯作者：
Pourcel, Christine
中科院分区：
文献类型：
--
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
聚集的定期间隔的短质体重复序列（CRISPR）构成了在广泛的原核生物基因组（Eubacteria的一半和几乎所有古细菌）中发现的特定串联重复段。 23至47个BP，通常是病毒起源的类似尺寸的独特序列（间隔）。在这里，我们描述了一个基于RNA的免疫系统。获取侧翼序列来确定（IV）爆炸垫片，以检查GenBank数据库，并且（V）在原核生物测序基因组中是否在其他地方找到了DR。服务器/CRISPRFINDER.PHP。
Clustered regularly interspaced short palindromic repeats (CRISPRs) constitute a particular family of tandem repeats found in a wide range of prokaryotic genomes (half of eubacteria and almost all archaea). They consist of a succession of highly conserved regions (DR) varying in size from 23 to 47 bp, separated by similarly sized unique sequences (spacer) of usually viral origin. A CRISPR cluster is flanked on one side by an AT-rich sequence called the leader and assumed to be a transcriptional promoter. Recent studies suggest that this structure represents a putative RNA-interference-based immune system. Here we describe CRISPRFinder, a web service offering tools to (i) detect CRISPRs including the shortest ones (one or two motifs); (ii) define DRs and extract spacers; (iii) get the flanking sequences to determine the leader; (iv) blast spacers against Genbank database and (v) check if the DR is found elsewhere in prokaryotic sequenced genomes. CRISPRFinder is freely accessible at http://crispr.u-psud.fr/Server/CRISPRfinder.php.