INHIBITION OF ICE FAMILY PROTEASES BY BACULOVIRUS ANTIAPOPTOTIC PROTEIN P35
INHIBITION OF ICE FAMILY PROTEASES BY BACULOVIRUS ANTIAPOPTOTIC PROTEIN P35
复制标题
DOI：
10.1126/science.7569933
复制
发表时间：
1995-09-29
期刊：
影响因子：
56.9
通讯作者：
WONG, WW
中科院分区：
文献类型：
--
作者：
BUMP, NJ;HACKETT, M;WONG, WW
杆状病毒抗凋亡蛋白p35抑制人白细胞介素-1 β转换酶（ICD）和它的同源物在酶促测定的蛋白水解活性。p35的共表达阻止了ICE的自蛋白水解激活，阻断了ICE诱导的细胞凋亡。酶活性的抑制与p35的切割和稳定的ICE-p35复合物的形成有关。p35在不同途径和远亲生物体中阻断细胞凋亡的能力表明ICE样蛋白酶在诱导细胞凋亡中的核心和保守作用。
The baculovirus antiapoptotic protein p35 inhibited the proteolytic activity of human interleukin-1 beta converting enzyme (ICD) and three of its homologs in enzymatic assays. Coexpression of p35 prevented the autoproteolytic activation on ICE from its precursor form and blocked ICE-induced apoptosis, Inhibition of enzymatic activity correlated with the cleavage of p35 and the formation of a stable ICE-p35 complex. The ability of p35 to block apoptosis in different pathways and in distantly related organisms suggests a central and conserved role for ICE-like proteases in the induction of apoptosis.