Structure and Functions of Tobacco Mosaic Virus RNA

Structure and Functions of Tobacco Mosaic Virus RNA
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烟草花叶病毒RNA的结构和功能

DOI:
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发表时间:
1990
期刊:
影响因子:
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通讯作者:
Yuichiro Watanabe
Yuichiro Watanabe
中科院分区:
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文献类型:
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作者:
Y. Okada;T. Meshi;Yuichiro Watanabe

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烟草花叶病毒(Tobacco mosaic virus,TMV)是一种特征性很强的植物病毒。TMV的基因组是正义单链RNA,并且编码至少三种非结构蛋白(130 K、180 K和30 K蛋白)和外壳蛋白(Goelet et al. 1982; Ohno等人,1984年)。非结构蛋白的功能在分子水平上还没有很好的理解。最近,已经建立了允许从克隆的全长cDNA拷贝产生感染性TMV RNA的体外表达系统(Dawson等人,1986; Meshi等人,1986年),因此反向遗传学方法已成为可能的TMV研究。我们在体外构建了几种TMV突变体,以鉴定TMV编码蛋白和基因组RNA的功能。
Tobacco mosaic virus (TMV) is one of the well-characterized plant viruses. The genome of TMV is a positive-sense, single-stranded RNA and encodes at least three non-structural proteins (130K, 180K and 30K proteins) and a coat protein (Goelet et al., 1982; Ohno et al., 1984). The functions of non-structural proteins are not well understood at the molecular level. Recently, in vitro expression systems that allow production of infectious TMV RNAs from cloned full-length cDNA copies have been established (Dawson et al., 1986; Meshi et al., 1986) and as a result reverse genetics approaches have become possible for TMV research. We have constructed several kinds of TMV mutants in vitro to identify the function of TMV-coded proteins and the genomic RNA.
DOI: 10.1016/0022-2836(89)90491-9
发表时间: 1989-04-05
影响因子: 5.6
作者:
DREHER, TW;RAO, ALN;HALL, TC
通讯作者: HALL, TC