A Single Male Auditory Response Test to Quantify Auditory Behavioral Responses in Drosophila melanogaster
A Single Male Auditory Response Test to Quantify Auditory Behavioral Responses in Drosophila melanogaster
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量化果蝇听觉行为反应的单一雄性听觉反应测试
DOI：
10.1080/01677063.2019.1611805
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发表时间：
2019
影响因子：
1.9
通讯作者：
Azusa Kamikouchi
中科院分区：
文献类型：
--
作者：
Yuki Ishikawa;Natsuki Okamoto;Yusuke Yoneyama;Naoki Maeda;Azusa Kamikouchi
许多动物利用听觉信号与同种个体进行交流。在求爱期间，果蝇的雄性果蝇和相关物种通过振动翅膀发出由正弦和脉冲歌曲组成的求爱歌曲。脉搏鸣声会增加雌性的接受能力和雄性的求偶活动，表明它起到了性信号的作用。一种歌曲参数，即脉冲间隔（IPI），在密切相关的物种之间有所不同。 InD。黑腹果蝇，具有同种 IPI 的歌曲比异种歌曲诱导更强的行为反应，表明果蝇具有区分同种 IPI 的能力。传统上，果蝇对歌曲的反应是在分组条件下测量的，其中不能排除听觉以外的感官方式的影响。在这里，为了量化个体辨别同种歌曲的能力，我们系统地分析了单个雄性果蝇对各种参数声音的听觉反应。此外，我们将这种称为 SMART（单一雄性听觉反应测试）的方法应用于两个姐妹物种，以进行比较方法的潜在应用。通过量化 singleD 的运动活动。在暴露于声音期间，我们检测到黑腹雄性对脉冲歌曲的运动活动增加，但对白噪声或纯音没有反应。同种鸣叫比异种鸣叫引起更强的反应，并且触角接收器的消融严重抑制了运动的增加。 IPI 变化较小的脉冲歌曲会引起连续响应，而 IPI 变化较大的歌曲的响应往往会迅速衰减。这提供了果蝇区分 IPI 变异的第一个证据，这可能告知歌手的年龄和社会背景。姐妹种，D. Sechellia，表现出对脉冲歌曲的运动反应，而D. simulans 没有表现出任何行为反应。这表明引起这种行为反应的听觉和其他刺激在果蝇物种中是多样化的。
Many animals utilize auditory signals to communicate with conspecific individuals. During courtship, males of the fruit flyDrosophila melanogasterand related species produce a courtship song comprised of sine and pulse songs by vibrating their wings. The pulse song increases female receptivity and male courtship activity, indicating that it functions as a sexual signal. One song parameter, interpulse interval (IPI), varies among closely related species. InD. melanogaster, a song with a conspecific IPI induces a stronger behavioral response than heterospecific songs, indicating the ability of the flies to discriminate conspecific IPI. Traditionally, the fly’s response to the song is measured under grouped conditions, in which the effect of sensory modalities other than audition cannot be excluded. Here, to quantify the individual ability to discriminate a conspecific song, we systematically analyzed the auditory response of single male flies to sound with various parameters. Moreover, we applied this method, termed SMART (Single Male Auditory Response Test), to two sister species for potential application in a comparative approach. By quantifying the locomotor activity of singleD. melanogastermales during sound exposure, we detected increased locomotor activity in response to pulse songs, but not to white noise or pure tone. The conspecific song evoked stronger response than the heterospecific songs, and ablation of their antennal receivers severely suppressed the locomotor increase. A pulse song with a small IPI variation evoked a continuous response, while the response to songs with highly variable IPIs tends to be rapidly decayed. This provides the first evidence that fruit flies discriminate IPI variations, which possibly inform the age and social contexts of the singer. Sister species,D. sechellia, exhibited a locomotor response to pulse song, whileD. simulansexhibited no behavioral response. This suggests that auditory and other stimuli that elicit this behavioral response are diversified amongDrosophilaspecies.